135-139 Xu LAJP 4438:Xu
نویسندگان
چکیده
In this study, a simple, rapid and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method is described for determination of voriconazole in rat plasma samples using pirfenidone as the internal standard (IS) from pharmacokinetic assays. Sample preparation was accomplished through a simple protein precipitation with acetonitrile, and chromatographic separation was performed on an Acquity BEH C18 column (2.1 × 50 mm, 1.7 μm) with gradient profile at a flow of 0.4 mL/min. The linearity of this method was found to be within the concentration range of 10-5000 ng/mL for voriconazole in rat plasma. Only 3.0 min was needed for an analytical run. The method was applied to a pharmacokinetic study of voriconazole in rats. RESUMEN. En este estudio se describe un método de espectrometría de masas en tándem de cromatografía líquida de ultra desempeño (UPLC-MS / MS) simple, rápido y sensible para la determinación de voriconazol en muestras de plasma de rata usando pirfenidona como patrón interno (IS) a partir de ensayos farmacocinéticos. La preparación de la muestra se llevó a cabo a través de una sencilla precipitación de proteínas con acetonitrilo, y la separación cromatográfica se realizó en una columna Acquity BEH C18 (2,1 x 50 mm, 1,7 μm) con perfil de gradiente a un caudal de 0,4 mL/min. La linealidad del método está dentro del rango de 10 a 5.000 ng/mL para voriconazol en plasma de rata. Se necesitaron sólo 3,0 min para una serie de análisis. El método se aplicó a un estudio de farmacocinética de voriconazol en ratas.
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