Combined Lab-on-a-chip and Microarray Approach for Biomolecular Interaction Sensing Using Surface Plasmon Resonance Imaging

نویسندگان

  • G. Krishnamoorthy
  • J. B. Beusink
  • E. T. Carlen
  • S. Schlautmann
  • H. L. de Boer
  • A. van den Berg
چکیده

Surface plasmon resonance imaging (SPR) is a well-established label-free detection technique for real-time biomolecular interaction measurements. An integrated LOC sensing system with fluidic control for sample movement to specific locations on microarray surface in combination with SPR imaging is demonstrated by the measurements of human IgG and anti-IgG interactions from 24 patterned regions.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Proteomics-on-a-chip for Biomarker Discovery

In proteomics research still two-dimensional gel electrophoresis (2D-GE) is currently used for biomarker discovery. We applied free flow electrophoresis (FFE) separation technology combined with biomolecular interaction sensing using Surface Plasmon Resonance (SPR) imaging in an integrated proteomics-on-a-chip device as a proof of concept for biomarker discovery.

متن کامل

On-chip Electrokinetic Sample Focus- Ing for Microarray-based Biomolecular Interaction Assays

Imaging surface plasmon resonance (iSPR) is a label-free microarray based technique in which multiple biomolecular interactions can be monitored simultaneously. In this work, we present an integrated electrokinetic focusing microfluidic chip and iSPR system suitable for large-scale microarray applications.

متن کامل

Plasmonic interferometric sensor arrays for high-performance label-free biomolecular detection.

A plasmonic interferometric biosensor that consists of arrays of circular aperture-groove nanostructures patterned on a gold film for phase-sensitive biomolecular detection is demonstrated. The phase and amplitude of interfering surface plasmon polaritons (SPPs) in the proposed device can be effectively engineered by structural tuning, providing flexible and efficient control over the plasmon l...

متن کامل

Label-free Biomolecular Interaction Sensing on Microarrays Using Surface Plasmon Resonance Imaging

Cover design: " Bianca " (white) cover with the " heartbeat " of SPR on the front and SPRi result on the back. To the beauty of all the little things around us…. 6 Ratio-diluted ligand density assays for single analyte kinetic analysis .. 87

متن کامل

Optimization of Sensitivity and Stability of Gold/Silver bi-Layer Thin Films Used in Surface Plasmon Resonance Chips

The aim of this study is experimental assay of sensitivity and stability of a bimetallic silver/gold SPR sensor chip. This chip utilizes the sensitivity of the silver and the stability of the gold. Moreover, the Silver layer (instead of usual Cr or Ti layer) was used as an adhesive intermediate layer between the Gold layer and the glass substrate. The optimization of the Gold/Silver thickness u...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2007