Rapid communication: mapping of the titin (TTN) gene to pig chromosome 15.

Source and Description of Primers. The initial primers for the PCR were designed based on human DNA sequence (accession no. X92412; Kolmerer et al., 1996). The position of the forward and reverse primers corresponded to exon 3 and exon 5, respectively. These primers are expected to amplify a fragment of 1.93 kb from human DNA. A PCR fragment of 2.1 kb was amplified from porcine genomic DNA. Partial sequences were compared with the human sequence, which showed 90% (1,038/1,159 bp) nucleotide identity. The pig sequences (AF124849-50) were then used to design additional primers that amplified a 1.8-kb fragment of pig TTN and were used both for physical mapping using the somatic cell hybrid panel (SCHP) and for PCRRFLP analysis.