Suppression subtractive hybridization.

Generation of differentially regulated genes in Mycobacterium tuberculosis isolates from cerebrospinal fluid and respiratory secretions using suppression subtractive hybridization

Background Suppression Subtractive Hybridization (SSH) is an established method for separating DNA molecules that distinguish two closely related DNA samples, which has become a powerful method for generating subtracted cDNA libraries. In this study, we postulated that SSH could be used to identify significant genetic differences between Mycobacterium tuberculosis causing meningitis and M. tube...

Genetic differences between two strains of Xylella fastidiosa revealed by suppression subtractive hybridization.

Suppression subtractive hybridization was used to rapidly identify 18 gene differences between a citrus variegated chlorosis (CVC) strain and a Pierce's disease of grape (PD) strain of Xylella fastidiosa. The results were validated as being highly representative of actual differences by comparison of the completely sequenced genome of a CVC strain with that of a PD strain.

Suppression Subtractive Hybridization in Penaeid Prawns: An Approach in Identifying Diseases and Differentially Expressed Genes

Alteration in gene transcripts is related to a wide spectrum of physiological and pathological changes in cells. Therefore, the identification of differential gene expression can be used to understand biological processes at a molecular level [1-4]. Various techniques such as Expressed Sequence Tags (EST), differential display, Serial Analysis of Gene Expression (SAGE), cDNA microarray, and sub...

Identification of Immune Responsive Genes in Bovine Airway Using Suppression Subtractive Hybridization

Identification Leptospira santarosai serovar shermani specific sequences by suppression subtractive hybridization.

In Taiwan, leptospirosis is caused mainly by Leptospira santarosai serovar shermani. Suppression subtractive hybridization was employed to isolate DNA fragments present in pathogenic L. santarosai serovar shermani but absent in non-pathogenic L. biflexa serovar patoc. Analysis of 23 subtracted DNA clones revealed 25 gene fragments by BLASTX program. Eight clones showed similarity to transposase...