The bactericidal effect of a complement-independent antibody is osmolytic and specific to Borrelia.

Bactericidal action of a complement-independent antibody against relapsing fever Borrelia resides in its variable region.

A single chain variable fragment (scFv) of CB515, a complement-independent bactericidal monoclonal IgM against a relapsing fever Borrelia, was constructed to investigate the region wherein the unique bactericidal function resides. Monomeric CB515 scFv (26 kDa) was capable of binding its Ag on whole organisms and by immunoblot. This binding was shown to be species and serotype-specific to the 19...

Identification of Two Epitopes on the Outer Surface Protein A of the Lyme Disease Spirochete Borrelia burgdorferi

A murine IgM monoclonal antibody (MA-2C6) with κ-light chains directed against an antigenic determinant of outer surface protein A (OspA) of the Lyme disease spirochete, Borreliaburgdorferi, is produced. This antibody could bind specifically to OspA antigen of several isolates of B. burgdorferi, but not to the non-Lyme disease bacteria such as T. pallidum and B. hermsii. Antibody MA-2C6 was pur...

Effects of dietary Aloe vera on some specific and nonspecific immunity in the common carp (Cyprinus carpio)

In this study, the immunostimulatory effect of dietary Aloe vera crude extract was investigated in Cyprinus carpio. Three hundred fish were randomly divided into four groups. The first group was immunized with Aeromonas hydrophila bacterin (A.h) and was fed a diet contained 0.5% Aloe vera. The second group was immunized with A.h and fed a diet without Aloe vera. The third group was not immunize...

Borrelia Bactericidal IgM against a Relapsing Fever Generation of a Complement-Independent

Antibody-dependent cell-mediated cytotoxicity- and complement-dependent cytotoxicity-independent bactericidal activity of an IgG against Pseudomonas aeruginosa O6ad.

In addition to Ag recognition, some Abs are capable of killing target organisms in the absence of phagocytes and complement. In this study, we report that an anti-Pseudomonas aeruginosa O6ad LPS IgG(1), tobacco-expressed human S20 IgG(1) (te-hS20), as well as its recombinant Fab and single-chain variable fragment (scFv) fragments have cellular- and complement-independent bactericidal activity. ...