Genetic Transformation System for the Fungal Soybean Pathogen Cercospora kikuchii.
نویسندگان
چکیده
An altered beta-tubulin gene that confers resistance to the fungicide benomyl was isolated from a genomic library of a UV-induced mutant of Cercospora kikuchii and used as a selectable marker for transformation. The level of benomyl resistance conferred to the transformants was at least 150-fold greater than the intrinsic resistance of the C. kikuchii recipient protoplasts. In the majority of cases, the tubulin fragment was integrated at the native beta-tubulin locus, apparently by gene replacement or gene conversion. The frequency of transformation ranged from 0.2 to 6 transformants per mug of DNA, depending on the recipient strain. Transformation with linearized plasmid resulted in a higher frequency, without changing the type of integration event. Transformants were phenotypically stable after eight consecutive transfers on medium without benomyl. This is the first report of a genetic transformation system for a Cercospora species.
منابع مشابه
More Cercospora Species Infect Soybeans across the Americas than Meets the Eye
Diseases of soybean caused by Cercospora spp. are endemic throughout the world's soybean production regions. Species diversity in the genus Cercospora has been underestimated due to overdependence on morphological characteristics, symptoms, and host associations. Currently, only two species (Cercospora kikuchii and C. sojina) are recognized to infect soybean; C. kikuchii causes Cercospora leaf ...
متن کاملDraft Genome Sequence of Cercospora cf. sigesbeckiae, a Causal Agent of Cercospora Leaf Blight on Soybean
Cercospora cf. sigesbeckiae is an ascomycete fungal pathogen that infects various plants, including important agricultural commodities, such as soybean. Here, we report the first draft genome sequence and assembly of this pathogen.
متن کاملExpression in sugar beet of the introduced cercosporin toxin export (CFP) gene from <Emphasis Type="Italic">Cercospora kikuchii</Emphasis>, the causative organism of purple seed stain in soybean
The Cercospora kikuchii cercosporin export gene, CFP, introduced into Beta vulgaris L. by conjugation with Rhizobium radiobacter, was stably maintained during vegetative propagation as verified by PCR using primers specific for the CFP gene. Transcriptional expression of the CFP gene in leaves was determined by RT-PCR using CFP-specific primers. CFP protein was detected using Western analysis w...
متن کاملGenetic mapping of resistance to purple seed stain in PI 80837 soybean.
Purple seed stain (PSS) of soybean caused by Cercospora kikuchii is an important disease that reduces market grade and can affect seed germination and vigor. A single dominant gene was shown to confer PSS resistance in PI 80837. The objective of this research was to map the PSS resistance gene in PI 80837 using simple sequence repeat (SSR) markers. A cross was made between the PSS-susceptible c...
متن کاملSoybean Seed Health
Fig. 3. Wrinkled, white, moldy-appearing seed infected by Phomopsis spp. More than 30 fungi are seed-borne on soybeans. Some, such as Phomopsis spp., the cause of pod and stem blight disease, can severely reduce seed viability (fi g. 1). Others cause quality problems, such as purple seed stain, caused by Cercospora kikuchii, but have less severe effects on viability. However, most fungi that ar...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 57 10 شماره
صفحات -
تاریخ انتشار 1991