Multiplexed droplet Interface bilayer formation.

Electro-Coalescence of an Aqueous Droplet at an Oil–Water Interface with an Investigation of Secondary Droplets Formation

Adsorption Kinetics Dictate Monolayer Self-Assembly for Both Lipid-In and Lipid-Out Approaches to Droplet Interface Bilayer Formation.

The droplet interface bilayer (DIB)--a method to assemble planar lipid bilayer membranes between lipid-coated aqueous droplets--has gained popularity among researchers in many fields. Well-packed lipid monolayer on aqueous droplet-oil interfaces is a prerequisite for successfully assembling DIBs. Such monolayers can be achieved by two different techniques: "lipid-in", in which phospholipids in ...

Triggered release of molecules across droplet interface bilayer lipid membranes using photopolymerizable lipids.

A combination of nonpolymerizable phospholipids (DPPC or DPhPC) and a smaller amount of cross-linking photopolymerizable phospholipids (23:2 DiynePC) is incorporated in an unsupported artificial lipid bilayer formed using the droplet interface bilayer (DIB) approach. The DIB is formed by contacting lipid monolayer-coated aqueous droplets against each other in a dodecane-lipid medium. Cross-link...

Novel technologies for the formation of 2-D and 3-D droplet interface bilayer networks.

Droplet interface bilayer (DIB) networks have vast potential in the field of membrane biophysics, synthetic biology, and functional bio-electronics. However a technological bottleneck exists in network fabrication: existing methods are limited in terms of their automation, throughput, versatility, and ability to form well-defined 3-D networks. We have developed a series of novel and low-cost me...

Electronic Supporting Information Direct in situ measurement of specific capacitance, monolayer tension, and bilayer tension in a droplet interface bilayer

Direct in situ measurement of specific capacitance, monolayer tension, and bilayer tension in a droplet interface bilayer