Time-Resolved Fluorescent Immunochromatography of Aflatoxin B1 in Soybean Sauce: A Rapid and Sensitive Quantitative Analysis
نویسندگان
چکیده
Rapid and quantitative sensing of aflatoxin B1 with high sensitivity and specificity has drawn increased attention of studies investigating soybean sauce. A sensitive and rapid quantitative immunochromatographic sensing method was developed for the detection of aflatoxin B1 based on time-resolved fluorescence. It combines the advantages of time-resolved fluorescent sensing and immunochromatography. The dynamic range of a competitive and portable immunoassay was 0.3-10.0 µg·kg(-1), with a limit of detection (LOD) of 0.1 µg·kg(-1) and recoveries of 87.2%-114.3%, within 10 min. The results showed good correlation (R² > 0.99) between time-resolved fluorescent immunochromatographic strip test and high performance liquid chromatography (HPLC). Soybean sauce samples analyzed using time-resolved fluorescent immunochromatographic strip test revealed that 64.2% of samples contained aflatoxin B1 at levels ranging from 0.31 to 12.5 µg·kg(-1). The strip test is a rapid, sensitive, quantitative, and cost-effective on-site screening technique in food safety analysis.
منابع مشابه
Rapid On-Site Sensing Aflatoxin B1 in Food and Feed via a Chromatographic Time-Resolved Fluoroimmunoassay
Aflatoxin B1 poses grave threats to food and feed safety due to its strong carcinogenesis and toxicity, thus requiring ultrasensitive rapid on-site determination. Herein, a portable immunosensor based on chromatographic time-resolved fluoroimmunoassay was developed for sensitive and on-site determination of aflatoxin B1 in food and feed samples. Chromatographic time-resolved fluoroimmunoassay o...
متن کاملDevelopment of antibody-based microarray assay for quantitative detection of aflatoxin B1
BACKGROUND: Aflatoxin B1 (AFB1) is a toxic metaboliteproduced by Aspergillus species that contaminates a wide range ofagricultural products. OBJECTIVES: This study was designed todevelop a rapid and highly sensitive immunoassay method inmicroarray format for quantitative detection of AFB1 to evaluatethe potential of microarray platform for high-throughput screening,which can be beneficial in fo...
متن کاملHighly Sensitive FRET-Based Fluorescence Immunoassay for Detecting of Aflatoxin B1 Using Magnetic/Silica Core-Shell as a Signal Intensifier
Background: Recently, some new nanobiosensors using different nanoparticles or microarray systems for detection of mycotoxins have been designed . However, rapid, sensitive and early detection of aflatoxicosis would be very helpful to distinguish high-risk persons. Objectives: We report a highly sensitive competitive immunoassay using magnetic/silica core shell as a signal intensifier for the d...
متن کاملApplication of Dispersive Liquid-Liquid Microextraction to Determine Aflatoxin B1 in Tomato Paste Samples
Background: Aflatoxin B1 is a secondary toxic metabolite produced by several aspergillus species. This study was conducted to determine the presence of aflatoxin in tomato paste samples. Methods: A total of 30 tomato pastes were analyzed for Aflatoxin B1 contamination via HPLC-FLD. Analyte extraction was done by dispersive liquid-liquid microextraction. Results: The limit of detection (LOD) and...
متن کاملComparison of Betanodavirus diagnosis quality in Grouper and Sea bass by Rapid Diagnosis Kit, histopathology and Nested-PCR methods
Betanodavirus is the leading cause of viral mortality in fish worldwide and a major barrier to the spread of fisheries and aquaculture. Due to the increase in aquaculture in the Persian Gulf and the occurrence of some disease symptoms in farmed and wild fish in the region, screening of Betanodavirus-sensitive fish became important. Therefore, to prevent economic losses and reduce aquatic reserv...
متن کامل