The Effection of Different Culturing Proportion of Deep Sea Water(DSW) to Surface Sea Water(SSW) in Reductive Ability and Phenolic Compositions of Sargassum Cristaefolium

Characterized as rich mineral substances, low temperature, few bacteria, and stability with numerous implementation aspects on aquaculture, food, drinking, and leisure, the deep sea water (DSW) development has become a new industry in the world. It has been report that marine algae contain various biologically active compounds. This research focued on the affections in cultivating Sagrassum cristaefolium with different concentration of deep sea water(DSW) and surface sea water(SSW). After two and four weeks, the total phenolic contents were compared in Sagrassum cristaefolium culturing with different ways, and the reductive activity of them was also be tried with potassium ferricyanide. Those fresh seaweeds were dried with oven and were ground to powder. Progressively, the marine algae we cultured was extracted by water under the condition with heating them at 90°C for 1hr.The total phenolic contents were be executed using Folin–Ciocalteu method. The results were explaining as follows: the highest total phenolic contents and the best reductive ability of all could be observed on the 1/4 proportion of DSW to SSW culturing in two weeks. Furthermore, the 1/2 proportion of DSW to SSW also showed good reductive ability and plentiful phenolic compositions. Finally, we confirmed that difference proportion of DSW and SSW is the major point relating to ether the total phenolic components or the reductive ability in the Sagrassum cristaefolium. In the future, we will use this way to mass production the marine algae or other micro algae on industry applications. Keywords—deep sea water(DSW), surface sea water(SSW), phenolic contents, reductive ability.