Reversed Phase Chromatography; Principles and methods handbook

Reversed Phase Chromatography; Principles and methods handbook

The Handbook of Analysis and Purification of Peptides and Proteins by Reversed-Phase HPLC

A simple, sensitive and rapid isocratic reversed-phase high-performance liquid chromatography method for determination and stability study of curcumin in pharmaceutical samples

Objective: This study was designed to develop and validate a new reversed-phase high-performance liquid chromatography (RP-HPLC) method based on Q2 (R1) International Conference on Harmonization (ICH) guideline for determination of curcumin in pharmaceutical samples. Materials and Methods: The HPLC instrument method was optimized with isocratic elution with acetonitrile: ammonium acetate (45:55...

EVALUATION OF THE USE OF ANIONIC/ NONIONIC MIXED MICELLES IN REVERSED PHASE LIQUID CHROMATOGRAPHY OF CHLOROPHENOLS

The use of aqueous mixed micellar system consisting of sodium dodecyl sulfate (SDS) and polyoxyethylene (23) dodecanol (Brij-35) as the mobile phase in reversed-phase liquid chromatography was studied. A group of chlorophenols was used as the test mixture. Adding organic modifier to the system showed that the use of low concentrations of organic additives improves efficiency in SDS/Brij-35 mixe...

Restricted-access Media: Solid-phase Extraction

reversed-phase (ISRP) material. Proteins do not adsorb on the hydrophilic exterior surfaces and do not penetrate into the hydrophobic interior surfaces, while analytes can reach the interior surfaces and be separated. (Reproduced with permission from Perry JA (1991) The internal surface reversed phase. Concept and applications. Journal of Liquid Chromatography 13: 1047}1074.) Gelfferich F (1962...