Ruxolitinib changes the natural course of myelofibrosis and its transplant outcome

which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Ruxolitinib changes the natural course of myelofibrosis and its transplant outcome THE AUTHORÊS REPLY: In his letter referring to " Will JAK1/2 inhibitors change the standard of care for myelofibrosis (MF)? " , Dr. Raut raised two important issues about rux-olitinib: (1) its mechanism of action and (2) its beneficial effect on the outcome of hematopoietic stem cell trans-plantation (HSCT) in MF patients. The first issue was about how ruxolitinib improved survival without causing any significant effect on disease biology parameters such as the peripheral blast count, marrow fibrosis, cytogenetic response , and JAK2 V617F allele burden. At the last annual meeting of the American Society of Hematology, long-term outcome data of COMFORT-I and COMFORT-II trials that either showed significantly improved survival of ruxolitinib group compared to placebo (hazard ratio (HR) 0.58, 95% confidence interval (CI) 0.36–0.95) or best supportive care (HR 0.52, 95% CI, 0.27–1.00) were presented. Since peripheral blast counts, marrow fibrosis, and cytogenetics were not investigated in those trials, it is difficult to explain the survival advantage on those parameters. However, with the use of ruxolitinib, the two studies showed significant sustained reduction of the JAK2 V617F allele burden. In COMFORT-I trials, ruxolitinib resulted in reduction of JAK2 mutant allele burden by a mean of 10.9% at week 24 (P ＜0.0001) and-21.5% at week 48 (P ＜0.0002) that were independent of baseline JAK2 V617F levels [1]. COMFORT-II trials also showed significant reduction in allele burden (median reduction rate of-7.0% at 48 weeks and-8.7% at 72 weeks), which was associated with spleen response [2]. Verstovsek et al. reported that 50% reduction in palpable spleen length was associated with prolonged survival in ruxolitinib treated patients [3]. These observations suggest that ruxolitinib could alter MF prognosis through reduction of JAK2 V617F allele burden and spleen volume. However, we should note that survival improvement was not great and longer follow-ups are required. The relationship between JAK2 V617F allele burden and the survival is also unclear. The second issue was regarding pre-transplant ruxolitinib and the improvement of HSCT outcome by decreasing proin-flammatory cytokines. Several convergent lines of evidence suggested that inflammatory cytokines act as mediators of acute GVHD and cause debilitating symptoms in MF. Ruxol-itinib significantly decreased tumor necrosis factor-alpha and interleukin-6 levels for a prolonged time in patients with MF through anti-JAK1-mediated downregulation …