Massively parallel recording of unit and local field potentials with silicon-based electrodes.

نویسندگان

  • Jozsef Csicsvari
  • Darrell A Henze
  • Brian Jamieson
  • Kenneth D Harris
  • Anton Sirota
  • Péter Barthó
  • Kensall D Wise
  • György Buzsáki
چکیده

Parallel recording of neuronal activity in the behaving animal is a prerequisite for our understanding of neuronal representation and storage of information. Here we describe the development of micro-machined silicon microelectrode arrays for unit and local field recordings. The two-dimensional probes with 96 or 64 recording sites provided high-density recording of unit and field activity with minimal tissue displacement or damage. The on-chip active circuit eliminated movement and other artifacts and greatly reduced the weight of the headgear. The precise geometry of the recording tips allowed for the estimation of the spatial location of the recorded neurons and for high-resolution estimation of extracellular current source density. Action potentials could be simultaneously recorded from the soma and dendrites of the same neurons. Silicon technology is a promising approach for high-density, high-resolution sampling of neuronal activity in both basic research and prosthetic devices.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Improvement of a Nano-scale Silicon on Insulator Field Effect Transistor Performance using Electrode, Doping and Buried Oxide Engineering

In this work, a novel Silicon on Insulator (SOI) MOSFET is proposed and investigated. The drain and source electrode structures are optimized to enhance ON-current while global device temperature and hot carrier injection are decreased. In addition, to create an effective heat passage from channel to outside of the device, a silicon region has embedded in the buried oxide. In order to reduce th...

متن کامل

NeuroResource Monolithically Integrated m LEDs on Silicon Neural Probes for High-Resolution Optogenetic Studies in Behaving Animals

Highlights d Multiple mLEDs and recording sites were fabricated monolithically on silicon d Spikes were robustly induced using ultra-low optical power (60 nW) d Neurons 50 mm apart were controlled independently in CA1 of freely moving mice d Deep and superficial parts of CA pyramidal layer form distinct ripple generators In Brief Recording and stimulating multiple individual neurons is critical...

متن کامل

Online analysis of local field potentials for seizure detection in freely moving rats

Objective(s): Seizure detection during online recording of electrophysiological parameters is very important in epileptic patients. In the present study, online analysis of field potential recordings was used for detecting spontaneous seizures in epileptic animals.Materials and Methods: Epilepsy was induced in rats by pilocarpine injecti...

متن کامل

Unit activity, evoked potentials and slow waves in the rat hippocampus and olfactory bulb recorded with a 24-channel microelectrode.

Activity from a number of neighboring neurons can be recorded simultaneously with multichannel microelectrodes. A new version of a 24-channel microelectrode system has been fabricated and used to record different types of neurophysiological data in the rat brain. The system called PRONG (Parallel Recording Of Neural Groups) includes a microelectrode, a lightweight reusable connector, a 24-chann...

متن کامل

Recording and marking with silicon multichannel electrodes.

This protocol describes an implementation of recording and analysis of evoked potentials in the hippocampal cortex, combined with lesioning using multichannel silicon probes. Multichannel recording offers the advantage of capturing a potential field at one instant in time. The potentials are then subjected to current source density (CSD) analysis, to reveal the layer-by-layer current sources an...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of neurophysiology

دوره 90 2  شماره 

صفحات  -

تاریخ انتشار 2003