Fully Integrated Rotary Genetic Analysis System
نویسندگان
چکیده
In this study, a fully integrated and portable Rotary Genetic Analyzer has been developed to analyze the gene expression of influenza A v irus . The Rotary Genetic Analyzer is made up of four parts : a disposable microchip, three thermal blocks for temperature control, a servo-motor for precise and high-rate spinning of the chip, and a miniaturized optical fluorescence detector. The microdevice was designed for performing microglass bead-based RNA template purification and then Rotary reverse transcriptase polymerase chain reaction (RT-PCR). Target gene amplification was real-time monitored using the integrated Rotary Genetic Analyzer system.
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