Cellular imaging: a key phenotypic screening strategy for predictive toxicology
نویسنده
چکیده
Incorporating phenotypic screening as a key strategy enhances predictivity and translatability of drug discovery efforts. Cellular imaging serves as a "phenotypic anchor" to identify important toxicologic pathology that encompasses an array of underlying mechanisms, thus provides an effective means to reduce drug development failures due to insufficient safety. This mini-review highlights the latest advances in hepatotoxicity, cardiotoxicity, and genetic toxicity tests that utilized cellular imaging as a screening strategy, and recommends path forward for further improvement.
منابع مشابه
Cellular imaging predictions of clinical drug-induced liver injury.
Drug-induced liver injury (DILI) is the most common adverse event causing drug nonapprovals and drug withdrawals. Using drugs as test agents and measuring a panel of cellular phenotypes that are directly linked to key mechanisms of hepatotoxicity, we have developed an in vitro testing strategy that is predictive of many clinical outcomes of DILI. Mitochondrial damage, oxidative stress, and intr...
متن کاملConcept Clearance for Engineered Tissue Systems for the Environmental Health Sciences
This program is intended to foster the development of in vitro experimental and computational models replicating major organ systems to be used for increased throughput and high data content screening of the mechanistic and toxicological effects of potential environmental toxicants. The proposed model systems will incorporate the inherent complexity of biology including the interactions between...
متن کاملI-41: Imaging Strategy for Diagnosis of Adnexal Masses Including US,CT Scan and MRI
Adnexal masses are spectrum of diseases from benign non neoplastic and neoplastic masses to malignant neoplasms. Endovaginal ultrasonography (US) is the fisrt and invaluable modality for assessment of adnexal masses because it is available and has a high negative predictive value. Important morphologic features are solid (vascularized) tissue, vascular and thick septations, and papillary projec...
متن کاملRobust Microplate-Based Methods for Culturing and in Vivo Phenotypic Screening of Chlamydomonas reinhardtii
Chlamydomonas reinhardtii (Cr), a unicellular alga, is routinely utilized to study photosynthetic biochemistry, ciliary motility, and cellular reproduction. Its minimal culture requirements, unicellular morphology, and ease of transformation have made it a popular model system. Despite its relatively slow doubling time, compared with many bacteria, it is an ideal eukaryotic system for microplat...
متن کاملAccelerated Cytotoxicity Mechanism Screening
By discovering how chemical compounds/xenobiotics cytotoxicity is affected when their metabolic pathways are inhibited or activated, the metabolic pathways that activate versus detoxify chemical compound can be identified. Reactive metabolites contributing to cytotoxicity can also be identified. In this lecture, the pretreatment of inhibitors and activators of xenobiotic metabolizing enzymes as...
متن کامل