Lysozyme-mediated formation of protein-silica nano-composites for biosensing applications.

نویسندگان

  • Madhumati Ramanathan
  • Heather R Luckarift
  • Ainur Sarsenova
  • James R Wild
  • Erlan K Ramanculov
  • Eric V Olsen
  • Aleksandr L Simonian
چکیده

We demonstrate a rapid method for enzyme immobilization directly on a waveguide surface by encapsulation in a silica matrix. Organophosphate hydrolase (OPH), an enzyme that catalytically hydrolyzes organophosphates, was used as a model enzyme to demonstrate the utility of lysozyme-mediated silica formation for enzyme stabilization. Silica morphology and the efficiency of OPH encapsulation were directly influenced by the precursor choice used in silica formation. Covalent attachment of the lysozyme template directly to the waveguide surface provided a stable basis for silica formation and significantly increased the surface area for OPH encapsulation. OPH conjugated to a pH-responsive fluorophore was encapsulated in silica and patterned to a waveguide surface to demonstrate the immobilization strategy for the development of an organophosphate array biodetector. Silica-encapsulated OPH retained its catalytic activity for nearly 60 days with a detection limit of paraoxon of approximately 35 microM. The encapsulation technique provides a potentially versatile tool with specific application to biosensor development.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Lysozyme-mediated Formation of Protein-silica Nano-composites for Biosensing Applications (postprint)

We demonstrate a rapid method for enzyme immobilization directly on a waveguide surface by encapsulation in a silica matrix. Organophosphate hydrolase (OPH), an enzyme that catalytically hydrolyzes organophosphates, was used as a model enzyme to demonstrate the utility of lysozyme-mediated silica formation for enzyme stabilization. Silica morphology and the efficiency of OPH encapsulation were ...

متن کامل

Influential Mechanisms and Potential Applications of Nano-Silicas in Cement Composites

Nanosilicas have been known as super-pozzolanic materials and compared to silica fume, these materials have higher purity of silica and finer particles. However, in contrast to silica fume, nanosilicas are produced by various methods, leading to different morphologies and agglomeration statuses in their initial form. On the other hand, due to several mechanisms such as the bridging effect of ca...

متن کامل

Synthesis and Characterization of Novel Modified and Functionalized Silica Nano-particles for Protein Delivery Applications

In this study, the synthesis, characterization and controlled release behavior of new Hollow Silica Nano particles (HSNPs) and Magnetic Silica Nano Particles (MSNPs) were studied. Magnetic Silica Nano particles (MSNPs), as drug delivery vehicles, were synthesized through the coating of Fe3O4 nano-crystals with silica layers. The HSNPs were obtained by removal of Fe3O4 templates with hydrochlori...

متن کامل

Enzyme Entrapment in Silica Nanoparticles: Mediated by Single Walled Carbon Nanotubes-lysozyme for Biosensor Applications

Introduction Silica compounds are among the most extensively studied materials, especially those prepared by the sol−gel process [13]. A wide variety of proteins can catalyze the precipitation of silica and become encapsulated as the silica matrix forms. The reaction provides an efficient method for enzyme immobilization and provides significant mechanical stability to resulting silica matrix. ...

متن کامل

Formation of Silica-Lysozyme Composites Through Co-Precipitation and Adsorption

1 Cohen Geochemistry Group, Earth Surface Science Institute, School of Earth and Environment, Faculty of Environment, University of Leeds, Leeds, United Kingdom, 2 Rock-Water Interaction Group, Institute of Geological Sciences, University of Bern, Bern, Switzerland, 3 Interface Geochemistry, German Research Centre for Geosciences, Potsdam, Germany, 4 Nano-Science Centre, Department of Chemistry...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Colloids and surfaces. B, Biointerfaces

دوره 73 1  شماره 

صفحات  -

تاریخ انتشار 2009