Evidence that Fgf10 offers therapeutic opportunities after hyperoxic lung injury in mice

Methods 1) 10 weeks old Fgf10 mice (50% Fgf10 expression compared to WT) in normoxic condition: Lung function and morphometric analysis. 2) BPD model: a) Fgf10 and Fgf10 mice were exposed to 85% O2 from P0-P8. Morphometric analysis and a-Actin/vWF staining were performed at P3. b) Rosa26;tet(O)Fgf10 (gain-of-function) mice were exposed to 85% O2 from P0-P8. From P9-P45 the pups were exposed to normoxia and fed either with normal food (control) or doxycycline food (experimental) to activate the transgene Fgf10. Morphometric analysis was carried out at P45. 3) Tolerance study: Rosa26;tet(O)Fgf10 and WT mice (both 10 weeks old) were exposed to doxycycline for 2 weeks. Then survival rate, histology, Ki67 and TUNEL staining were performed.