An ultrastructural autoradiographic study of the incorporation of (35S)cystine in the wool fibre cortex.

Incorporation of [Me-3H]methionine into wool follicle proteins: a biochemical and ultrastructural study.

The formation of the 'low-sulphur' group of proteins which constitutes more than half the cortical cell content of wool was investigated. Of the amino acids present in the lowand highsulphur proteins isolated from wool, methionine is the only one which is exclusive to the low-S group. The incorporation of [Me-'H]methionine, administered intradermally to a sheep, was examined using both biochemi...

An ultrastructural study of the membranes of keratinizing wool follicle cells.

Measurements of the widths of apposed plasma membranes and of the spaces between them were made at different stages of differentiation of keratinizing and hardening cells of the wool follicle. In contrast to findings in the hair follicle, no changes were detected for apposed cortex, cortex/fibre cuticle and fibre cuticle/fibre cuticle cells until keratinization had taken place. The trilaminar a...

The effect of increasing methionine supply on the methionine conversion to cyst(e)ine in sheep.

The conversion of methionine to cyst(e)ine was determined in sheep infused with different amounts of methionine (0-5 g/d) into the duodenum by assaying the incorporation of 35S from intravenously-infused L-[35S]methionine into cyst(e)ine in wool, plasma albumin and the free plasma pool. The percentage of cystine-S in the plasma originating from methionine increased linearly from 4.5 to 18 with ...

Ultrastructural autoradiographic study of the uptake and intracellular localization of 35S-sulfate by developing human neutrophils.

The Growth and Composition of Wool following Defleecing of Sheep with N-[5-(4-aminophenoxy)pentyl]phthalimide

In four sheep dosed with N-[5-(4-aminophenoxy)pentyl]phthalimide (400 mg/kg live weight) fibre growth stopped within l-ls days and the sheep were readily defleeced after 9 days. Most follicles stopped growing fibres for 12-16 days. When wool growth resumed, autoradiographic measurements indicated that it was slightly enhanced in the early regrowth due to an increase in fibre diameter. This incr...