Chromosomal Localization of Repetitive Dna in the Newt, Triturus
نویسندگان
چکیده
The repetitive DNA sequences of the newt, Triturus viridescens, have been studied by nucleic acid hybridization procedures. Complementary RNA was synthesized enzymatically from unfractionated newt DNA. This RNA hybridized strongly to the centromeric regions of both somatic and lampbrush chromosomes It also bound to other loci scattered along the lengths of the chromosomes The amplified ribosomal DNA in the multiple oocyte nucleoli was demonstrated by in situ hybridization
منابع مشابه
Variability of DNA methylation pattern in somatic and germ cells in male newt (Amphibia, Urodela) Triturus cristatus carnifex.
In a survey of several mammalian genomes, namely humans, rodents and bovines, the differences in the 5-methylcytosine (m5C) content show that repeated DNA sequences from sperm were undermethylated and from various somatic tissues were heavily methylated. This report shows a pattern of methylation in male newt (Amphibia, Urodela) Triturus cristatus carnifex (T. c. c.) unlike that so far describe...
متن کاملThe Occurrence and Possible Significance of Haemoglobin in the Chromosomal Regions of Mature Erythrocyte Nuclei of the Newt Triturus Cristatus Cristatus
Absorption measurements in violet light (lambda 4047 A) of thin sections of mature newt erythrocytes have indicated the presence of a haem compound, probably haemoglobin, within the chromosomal regions of the nucleus. The amount is about 45 per cent of that in the cytoplasm. The possible significance of this nuclear haemoglobin in determining nuclear structure is discussed in terms of the elect...
متن کاملThe application of eDNA for monitoring of the Great Crested Newt in the UK
Current ecological surveys for great crested newts are time-consuming and expensive and can only be carried out within a short survey window. Additional survey methods which would facilitate the detection of rare or protected species such as the great crested newt (Triturus cristatus) would be extremely advantageous. Environmental DNA (eDNA) analysis has been utilized for the detection of great...
متن کاملAn experimental analysis of bivalent interlocking in spermatocytes of the new Triturus vulgaris.
Interlocked bivalents at 1st meiotic metaphase are relatively uncommon in spermatocytes of the newt Triturus vulgaris, but their frequency of occurrence can be significantly increased by subjecting newts to a 24-h heat shock. Newt spermatocytes are sensitive to a heart shock at any stage between the end of premeiotic S and mid to late pachytene. The heat shock does not cause evidence desynapsis...
متن کاملSPHEROIDAL AND RING NUCLEOLI IN AMPHIBIAN OOCYTES Patterns of Uridine Incorporation and Fine Structural Features
In maturing oocytes of the newt Triturus viridescens, the nucleoli undergo a series of morphological changes that are very similar to those described by Callan for the axolotl, Ambystoma mexicanum. The nucleoli first assume the form of spheroids which then become extended into ring or necklace shapes that are DNase-sensitive; in mature oocytes the nucleoli revert to a spheroidal form. Short ter...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of Cell Biology
دوره 54 شماره
صفحات -
تاریخ انتشار 1972