Screening the effective constituent of perilla compound prescription on anti-motion sickness in mice model

Motion sickness will not be life-threatening, but it will bring a lot of inconvenience for daily life, also reducing combat effectiveness of military troops or affecting the health and work efficiency of astronauts. In the article, a more effective mice motion sickness model which selected kaolin consumption, food intake and halo response index as evaluation indexes was firstly established. Then n-butanol extract of perilla compound prescription (including medicinal and homologous plants named perilla, zingiber, pericarpium citri reticulatae and mint) was defied as effective chemical part to improve mice’s evaluation indexes of motion sickness mentioned above, at the same time reducing their AVP levels and increasing their CORT levels to alleviate the mice’s symptoms of motion sickness. What’s more, the anti-motion sickness activity of n-butanol constituent was attributed to four multi-component substances (water, 25% ethanol, 65% ethanol and 95% ethanol elution parts). After that, MPLC, silica gel column chromatography, TLC and HPLC-MS were also used to analyze the main compounds of the n-butanol effective constituent. Three compounds of n-butanol extract were identified, one of them is Pachyaximine A, which has potential cholinolytic function related with motion sickness. But, the n-butanol extract can only play a short-term effect on motion sickness. In order to offer a long-term effect, a kind of sustained-release membrane was made. Its transdermal absorption behavior conformed to the Higuchi equation, which was Qn = 94.46t1/2+170.42. And its accumulated transmittance η could reach 78.97% after 24 h, indicating a good sustained-release effect to prolong effective time of preventing motion sickness in mice.