Isolation of a benz(a)pyrene-thymine photoadduct from DNA hydrolized after irradiation at 365 nm in the presence of benz(a)pyrene.
نویسندگان
چکیده
The photoreaction which takes place between benz [a] pyrene and DNA by irradiation at 365 nm leads to a covalent combination. To ascertain the reactive sites of DNA, a sample of the macro molecule has been precipitated after irradiation in the presence of benz [a] pyrene and hydrolized; among the products of hydrolysis four different fluorescent substances have been isolated, one of which was identical with the photoadduct benz[a]pyrene-thymine, already prepared by irradiation of the two substances at 365 nm. This photoadduct has been obtained in an amount corresponding to a molecular ratio 1 : 7000 in respect to the nucleotides (P atoms) present in the sample of DNA.
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ورودعنوان ژورنال:
- Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete
دوره 25 11 شماره
صفحات -
تاریخ انتشار 1970