Oxytocin-induced labor augments IL-1 -stimulated lung fluid absorption in fetal guinea pig lungs
نویسندگان
چکیده
Nair, Prem K., Tianbo Li, Reshma Bhattacharjee, Xin Ye, and Hans G. Folkesson. Oxytocin-induced labor augments IL-1 -stimulated lung fluid absorption in fetal guinea pig lungs. Am J Physiol Lung Cell Mol Physiol 289: L1029–L1038, 2005. First published August 5, 2005; doi:10.1152/ajplung.00256.2004.—We tested the hypothesis that oxytocin-induced labor augmented IL-1 -induced/ -stimulated lung fluid absorption in preterm guinea pig fetuses. IL-1 was administered subcutaneously daily to timed-pregnant guinea pigs for 3 days with and without simultaneous cortisol synthesis inhibition by metyrapone. At day 3, oxytocin was administered, and fetuses were delivered by abdominal hysterotomy at 61 and by oxytocin-induced birth at 68 days gestation. Delivered fetuses were instilled with isosmolar 5% albumin into the lungs, and lung fluid movement was measured over 1 h by mass balance. Lung fluid absorption was induced in 61-day and stimulated in 68-day gestation lungs by IL-1 . Labor induction by oxytocin augmented IL-1 -induced/-stimulated lung fluid absorption. Metyrapone pretreatment did not affect oxytocin-induced/-stimulated lung fluid absorption, while completely blocking IL-1 -induced/-stimulated fluid absorption. Fetal lung fluid absorption, when present, was always propranolol and amiloride sensitive, suggesting that -adrenoceptor stimulation and amiloridesensitive sodium channels were critical for fluid absorption. Epithelial sodium channel and Na-K-ATPase subunit expressions were both increased by IL-1 , but not further by oxytocin. Our results indicate that IL-1 release into the maternal blood circulation positively affects lung maturation due to the IL-1 -induced release of cortisol and thus prepares the lungs for the epinephrine surge associated with labor.
منابع مشابه
Oxytocin-induced labor augments IL-1beta-stimulated lung fluid absorption in fetal guinea pig lungs.
We tested the hypothesis that oxytocin-induced labor augmented IL-1beta-induced/-stimulated lung fluid absorption in preterm guinea pig fetuses. IL-1beta was administered subcutaneously daily to timed-pregnant guinea pigs for 3 days with and without simultaneous cortisol synthesis inhibition by metyrapone. At day 3, oxytocin was administered, and fetuses were delivered by abdominal hysterotomy ...
متن کاملA noninflammatory interleukin-1beta fragment stimulates fetal lung fluid absorption in guinea pigs.
We have previously demonstrated that full-length interleukin (IL)-1beta can induce and stimulate lung fluid absorption in near-term guinea pig fetuses via stimulation of fetal cortisol synthesis and release. To develop a potentially clinically useful drug, we tested the hypothesis that maternal administration of a noninflammatory IL-1beta-fragment (IL-1beta(Fr)) induced cortisol synthesis and s...
متن کاملA Noninflammatory Interleukin-1 Fragment Stimulates Fetal Lung Fluid Absorption in Guinea Pigs
We have previously demonstrated that full-length interleukin (IL)-1 can induce and stimulate lung fluid absorption in nearterm guinea pig fetuses via stimulation of fetal cortisol synthesis and release. To develop a potentially clinically useful drug, we tested the hypothesis that maternal administration of a noninflammatory IL-1 -fragment (IL-1 Fr) induced cortisol synthesis and stimulated lun...
متن کاملStimulation of MAP kinase pathways after maternal IL-1β exposure induces fetal lung fluid absorption in guinea pigs
BACKGROUND We tested the hypothesis that maternal interleukin-1beta (IL-1beta) pretreatment and induction of fetal cortisol synthesis activates MAP kinases and thereby affects lung fluid absorption in preterm guinea pigs. METHODS IL-1beta was administered subcutaneously daily to timed-pregnant guinea pigs for three days. Fetuses were obtained by abdominal hysterotomy and instilled with isosmo...
متن کاملAlveolar Epithelial Ion and Fluid Transport Ca -dependent stimulation of alveolar fluid clearance in near-term fetal guinea pigs
Norlin, Andreas, and Hans G. Folkesson. Ca2 -dependent stimulation of alveolar fluid clearance in near-term fetal guinea pigs. Am J Physiol Lung Cell Mol Physiol 282: L642–L649, 2002; 10.1152/ajplung.00417.2000.—We investigated the importance of changes in intracellular Ca2 concentration ([Ca2 ]i) for amiloride-sensitive alveolar fluid clearance (AFC) in late-gestational guinea pigs. Fetal guin...
متن کامل