Heterologous Expression of Laccase (LACP83) of Pleurotus ostreatus
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چکیده
The heterologous expression of the gene LACP83 (encoding a laccase) from Pleurotus ostreatus in Escherichia coli was characterized. The laccase enzyme activity and kinetics of bacterial growth with an inducer (IPTG) and without inducer were determined. The maximum enzymatic activity was observed at 7 h post induction with a value of 3740 ± 342 U/L, which was similar to that reported for the native strain of P. ostreatus at 144 h of culture. Furthermore, the induction of laccase with IPTG reduced the specific growth rate of recombinant E. coli BL21 by approximately 50%. These results support the use this system for the recombinant production of the enzyme on an industrial scale.
منابع مشابه
Description of a Laccase Gene from Pleurotus Ostreatus Expressed under Submerged Fermentation Conditions
In this work, a gene (lacP83) encoding a Pleurotus ostreatus laccase isoenzyme expressed in submerged fermentation conditions is described. A 2,887 bp sequence was obtained from a genomic library of P. ostreatus by using a PCR inverse strategy. The coding sequence, 1,527 bp long, showed 17 exons and encoded a protein of 509 amino acids, with a putative signal peptide and conserved copper bindin...
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