Nonspecific Esterases of the Formed Elements: Zymograms Produced by pH 9.5 Polyacrylamide Gel Electrophoresis
نویسنده
چکیده
The formed elements of human blood each contain multipIe isoenzymes of nonspecific esterase that hydrolyze short chain alpha naphthyl esters. Zymograms that are characteristic of each type of formed element are obtained by subjecting purified preparations of each to polyacrylamide slab gel electrophoresis at pH 9.5 and subsequent staining of the gels for esterase activity. The most prominent isoenzyme detected is a species of low mobility that is reactive with either acetyl or butyryl esters and is highly sensitive to inhibition by 40 mM sodium fluoride. Also detected are several major acetyl esterases and a single
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Nonspecific esterases of the formed elements: zymograms produced by pH 9.5 polyarcrylamide gel electrophoresis.
The formed elements of human blood each contain multiple isoenzymes of nonspecific esterase that hydrolyze short chain alpha naphthyl esters. Zymograms that are characteristic of each type of formed element are obtained by subjecting purified preparations of each to polyacrylamide slab gel electrophoresis at pH 9.5 and subsequent staining of the gels for esterase activity. The most prominent is...
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