Transformation of antibody of Japanese encephalitis from IGM to IGG in experimental infected hen and transmission of IGG from hen to chicks. (epidemiological study on Japanese encephalitis. 37).

Transformation of Japanese encephalitis antibody from IgM to IgG in the sera of the experimental infected chicks with Japanese encephalitis virus and transmission of IgM or IgG from hen to chicks were examined by the gel filtration on Sephadex G-200 column. The following results were obtained. 1. Titer of hemoagglutination inhibiting antibody rose on seven days after inoculation of mouse brain homogenate infected with Japanese encephalitis, and that increased rapidly after the second inoculation of Japanese encephalitis. The maximum peak of antibody titer attained on 35 days after the first inoculation, on 7 days after the second inoculation and it maintained for a period of 2 months then decreased. Viremia was detected till 6 hours after the first inoculation. 2. IgM antibody by gel filtration appeared on 7 days after the first inoculation, kept on rising, reached the peak on 35 days after the first inoculation, then decreased, and disappeared on 120 days. IgG antibody appeared about 2 weeks after the IgM antibody appearance, and the titer of IgG antibody became higher than that of IgM antibody on 35 days after the first inoculation, then decreased gradually, and showed 1 : 16 of titer of peak on 150 days by gel filtration. 3. We could obtain the chicks by fertilization from experimentally infected hen, having IgM and IgG of hemoagglutination inhibiting antibody of Japanese encephalitis. And the localization of antibodies in the sera of its chicks was determined by Sephadex G-200 gel filtration. And IgG antibody was detected in chick serum, though IgM antibody was not detected by this method. ∗PMID: 4334579 [PubMed indexed for MEDLINE] Copyright c ©OKAYAMA UNIVERSITY MEDICAL SCHOOL Acta Med. Okayama 24, 589-596 (1970) TRANSFORMATION OF ANTmODY OF JAPANESE ENCEPHALITIS FROM IGM TO IGG IN EXPERIMENTAL INFECTED HEN AND TRANSMISSION OF IGG FROM HEN TO CHICKS (EPIDEMIOLOGICAL STUDY ON JAPANESE ENCEPHALITIS 37) Masana OGATA*, Yutaka NAGAO*, Ritsuko KIKUI** and Naoji KITAMURA** * Department of Public Health, Okayama University Medical School ** Institute of Hygiene, Okayama Prefecture, Okayama, Japan Received for publication, November 9, 1970 The potential role of wild birds in the epidemiology ofJapanese encephalitis has been studied by experimental infection ofJapanese encephalitis. KITAOKA, et at. 0-2) and HAMMON, et at. (3) showed independently that virus regularly appeared in the blood, following inoculation of wild and domestic birds with Japanese encephalitis virus in low mouse passage. BUESCHER, et at. (4-5) described in detail about the time of onset, magnitude and duration of viremia of chicks. They also studied the antibody appearance of black-crowned night herons, little ergets and plumed ergets and concluded that the black-crowned night herons played the most important role among herons. They described that hemoag-glutinine inhibiting (HI) and neutralizing (NT) antibodies of hen infected experimentally and those of heron infected naturally transferred to chick as maternal antibody. We (6) recognized that sera of plumed egrets showed high ratio (41 %) of HI positive reaction and plumed egrets can also be one of the amplifier ofJapanese encephalitis. We (6) found the presence of naturally infected hens by HI reaction in 1967 and the positive rate was 7 out of 27, and 6 out of 7 positive serum showed 2-ME positive reaction, suggesting that hen was one of the infectious sources of Japanese encephalitis. The HI antibody of hen infected naturally was also transferred to chicks and disappeared in the chick serum after hatching, and half life of the chick HI antibody was four days (7). In this experiment, we investigated transformation of IgM to IgG in the serum of naturally infected hen, and also studied whether JgM or IgG transferred from hen to chick. This report described briefly our results.