One-tube restriction enzyme digest and fluorescent labeling for restriction endonuclease fingerprinting single-strand conformational polymorphism.
نویسندگان
چکیده
منابع مشابه
Ligation-mediated PCR amplification of specific fragments from a class-II restriction endonuclease total digest.
A method is described which permits the ligation- mediated PCR amplification of specific fragments from a Class-II restriction endonuclease total digest. Feasibility was tested using Bcl I and phage lambda DNA as a model enzyme and amplicon system, respectively. Bcl I is one of many widely used restriction enzymes which cleave at palindromic recognition sequences and leave 5'-protruding ends of...
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Restriction Site Mapping is one of the interesting tasks in Computational Biology. A DNA strand can be thought of as a string on the letters A, T, C, and G. When a particular restriction enzyme is added to a DNA solution, the DNA is cut at particular restriction sites. The goal of the restriction site mapping is to determine the location of every site for a given enzyme. In partial digest metho...
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Single-strand conformation polymorphism (SSCP) (4) is the most widely used DNA screening method. SSCP detects single-base sequence changes by abnormal electrophoretic migration of one or both single strands on a nondenaturing gel. The method does not detect all sequence changes, and the sensitivity is a complex function of sequence context and size (1). Dideoxy fingerprinting (ddF) and bi-direc...
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Type IIS restriction enzymes have been successfully used as "universal" restriction enzymes in DNA manipulations. We took a step further to develop a rapid technique for recombining DNA fragments, fully automatic single-tube recombination (FASTR), which enables multiple-fragment DNA recombination in a single step. Crude PCR products are directly mixed with both type IIS restriction endonuclease...
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We have developed an automated, high-throughput fingerprinting technique for large genomic DNA fragments suitable for the construction of physical maps of large genomes. In the technique described here, BAC DNA is isolated in a 96-well plate format and simultaneously digested with four 6-bp-recognizing restriction endonucleases that generate 3' recessed ends and one 4-bp-recognizing restriction...
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عنوان ژورنال:
- BioTechniques
دوره 37 6 شماره
صفحات -
تاریخ انتشار 2004