The value of the reprocessing method of paraffin-embedded biopsies for transmission electron microscopy.

نویسندگان

  • Rodica Lighezan
  • Flavia Baderca
  • Aurora Alexa
  • M Iacovliev
  • Diana Bonţe
  • Elena Doina Murărescu
  • Adriana Nebunu
چکیده

Transmission electron microscopy (TEM) implies an elaborate preparation protocol that includes: fixation in glutaraldehyde followed by osmium tetraoxide postfixation, specimen dehydration, infiltration, resin embedding, ultrathin sectioning and staining with heavy metal salts. The aim of TEM is to examine the ultrastructure of specimens in ways that cannot be examined using other equipments or techniques. In some cases, when the requirement for TEM were made after tissue collection, useful information can be obtained from reprocessing the formalin-fixed, wax-embedded tissue used for light microscopy.

متن کامل
منابع مشابه

Correlation between histopathological criteria of Human Papilloma Virus infection and the presence of HPV in paraffin-embedded cervical biopsies based on PCR assay

Introduction: This study was carried out to correlate histological findings in cervical lesions to human papilloma virus (HPV), as detected by polymerase chain reaction (PCR). Methods: In this descriptive study, out of 143 cervical biopsies, 50 biopsies, containing sufficient histopathologic criteria, were examined. PCR assay was performed with the primers GP05/06+ and, as control, the beta-glo...

متن کامل

Immunoelectron Microscopic Observation of Chicken Glucagon-Like Peptide (GLP)-1-Containing Cells in Tissues Derived from Thin Section, Paraffin Block and Conventional Method

The purpose of the present study was to investigate the possibility of immunoelectron microscopic observation of endocrine cells in paraffin-embedded tissues. The procedure, which involves reprocessing from sliced tissues and immunohistochemical staining by colloidal-gold immunolabeling of paraffin sections from paraffin blocks, was able to reveal the fine characteristics of secretory granules ...

متن کامل

Comparison of Polymerase Chain Reaction, Ziehl-Neelsen Staining and Histopathologic Findings in Formalin-fixed, Paraffin-Embedded Tissue Specimens for Diagnosis of Tuberculosis

Background and Objective: Tuberculosis is still a major health problem, involving about 1/3 of the world´s population. Diagnosis is difficult when we only use Ziehl-Neelson staining. Many cases may be missed. A more rapid and sensitive diagnostic method is necessary. PCR may be helpful. The aim of this study was to compare PCR, Zieh-Neelsen staining and histopathologic findings in diagnosis of ...

متن کامل

Transmission Electron Microscopy Sample Preparation of INCONEL 738 Nickel-Base Superalloy

Size, shape, volume fraction and distribution of embedded g/ phase in g phase has direct effect on strength of INCONEL alloy. Microstructure parameters of INCONEL phases are quantified from microstructure images using transmission electron microscopy (TEM). Different TEM sample preparation techniques were used to study INCONEL 738 alloy microstructure for transmission electron micros...

متن کامل

The Comparison of different Procedures for DNA extraction from paraffin-embedded Tissues: A commercial kit and a traditional method based on heating

Abstract Background and objectives: Paraffin-embedded tissues and clinical samples are a valuable resource for molecular genetic studies, but the extraction of high-quality genomic DNA from this tissues is still a problematic issue. In the Present study, the efficiency of two DNA extraction protocols, a commercial kit and a traditional method based on heating and K Proteinase was compared. Mate...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

متن کامل
عنوان ژورنال:
  • Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie

دوره 50 4  شماره 

صفحات  -

تاریخ انتشار 2009