A Role for the Tec Family Tyrosine Kinase Txk in T Cell Activation and Thymocyte Selection

نویسندگان

  • Connie L. Sommers
  • Ronald L. Rabin
  • Alexander Grinberg
  • Henry C. Tsay
  • Joshua Farber
  • Paul E. Love
چکیده

Recent data indicate that several members of the Tec family of protein tyrosine kinases function in antigen receptor signal transduction. Txk, a Tec family protein tyrosine kinase, is expressed in both immature and mature T cells and in mast cells. By overexpressing Txk in T cells throughout development, we found that Txk specifically augments the phospholipase C (PLC)-gamma1-mediated calcium signal transduction pathway upon T cell antigen receptor (TCR) engagement. Although Txk is structurally different from inducible T cell kinase (Itk), another Tec family member expressed in T cells, expression of the Txk transgene could partially rescue defects in positive selection and signaling in itk(-)(/)(-) mice. Conversely, in the itk(+/+) (wild-type) background, overexpression of Txk inhibited positive selection of TCR transgenic thymocytes, presumably due to induction of cell death. These results identify a role for Txk in TCR signal transduction, T cell development, and selection and suggest that the Tec family kinases Itk and Txk perform analogous functions.

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عنوان ژورنال:
  • The Journal of Experimental Medicine

دوره 190  شماره 

صفحات  -

تاریخ انتشار 1999