Amniotic Fluid Phospholipids
نویسنده
چکیده
568 CLINICAL CHEMISTRY, Vol. 29, No. 3, 1983 high-density lipoprotein for cholesterol determination: Ultracentrifugation vs precipitation with sodium phosphotungstate and magnesium chloride. Clin Chem 27, 838841 (1981). 7. Kohlmeier M, Schlierf G. Effect of storage on HDL-cholesterol measurements. Clin Chem 28, 396-397 (1982). Letter. 8. Demacker PNM, Hijmans AG, van Sommeren-Zondag DF, et al. Stability of frozen liquid control sera for assay of cholesterol in
منابع مشابه
Critical assessment of phospholipid measurement in amniotic fluid.
We assessed several methods of inorganic phosphate assay for their suitability in estimating phospholipids in digested extracts of amniotic fluids. The extraction and digestion procedures used for phospholipids from amniotic fluid were also examined critically. The effect of contamination by blood or obstetric cream has been examined. Accordingly, we suggest a method for measuring total phospho...
متن کاملFluorometric procedure for determining "lamellar body" phospholipids in the particulate fraction of amniotic fluid after filtration.
A rapid (30-min) semiautomated continuous-flow procedure is described for use in assessing the phospholipids of the particulate ("lamellar body") fraction of human amniotic fluid. The method is based on measuring the difference in fluorescence of 1,6,-diphenyl-1,3,5-hexatriene added to amniotic fluid before and after micropore filtration. The filtration step removes "lamellar body" particles, w...
متن کاملThe role of amniotic fluid phospholipids in determining fetal lung maturity.
Assessment of the status of amniotic fluid phospholipids (qualitative and semi-quantitative) has become important in determining fetal lung maturity. In uncomplicated pregnancies, it has been documented that the ratio of lecithin to sphingomyelin (L/S ratio), with respect to pediatric gestational age, is a reliable predictor of maturity. In complicated pregnancies (e.g., toxemia, diabetes melli...
متن کاملMicroviscosity of amniotic fluid phospholipids, and its importance in determining fetal lung maturity.
Fluorescence polarization measurements of microviscosity (apparent viscosity within the hydrophobic center of lipid bilayers) of amniotic fluid correlate well with lecithin/sphingomyelin ratios determined by thin-layer chromatography. In addition to lecithin, phosphatidylglycerol and phosphatidylinositol are important for determining fetal lung maturity, but the lecithin/sphingomyelin ratio giv...
متن کاملAmniotic fluid phospholipids measured by continuous-development thin-layer chromatography.
We describe a one-dimensional thin-layer chromatographic system for separation of amniotic fluid phosphatidylcholine, sphingomyelin, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, and phosphatidylethanolamine in amniotic fluid. We utilize short-bed continuous development and "high performance" thin-layer chromatography. Phospholipids are detected with an antimony molybdate stai...
متن کاملMeasurement of the ratio of lecithin to sphingomyelin in amniotic fluid by fast atom bombardment mass spectrometry.
We describe a fast atom bombardment mass spectrometric method to determine the ratio of lecithin to sphingomyelin in amniotic fluid. Between m/z 400 and 1000, amniotic fluid extracts showed clear peaks for only sphingomyelin and lecithin. The molecular species of fatty acid in the phospholipids were analyzed quantitatively and quickly in a small volume of the extracts. The dipalmitoyllecithin/p...
متن کامل