Site-specific inhibition of photophosphorylation in isolated spinach chloroplasts by HgCL2. II. Evidence for three sites of energy conservation associated with non-cyclic electron transport.
نویسندگان
چکیده
Energy transfer inhibition by HgCl2 has been demonstrated to be selective for certain System I partial reactions. On the basis of different HgCl2 effects on the System I reactions, reduced 2,6-dichlorophenolindophenol --> methylviologen, diaminodurene --> methylviologen and N-phenazine methosulfate cyclic, two sites of energy conservation associated with System I are proposed. Furthermore, these sites are in parallel with each other, in series with the site closely associated with Photosystem II and are shared between non-cyclic and cyclic electron transport.
منابع مشابه
Site-specific Inhibition of Photophosphorylation in Isolated Spinach Chloroplasts by Mercuric Chloride.
Photophosphorylation associated with noncyclic electron transport in isolated spinach (Spinacia oleracea) chloroplasts is inhibited to approximately 50% by low concentrations of HgCl(2) (less than 1 mumole Hg(2+)/mg chlorophyll) when the electron transport pathway includes both sites of energy coupling. Reactions involving only a part of the electron transport system can give a functional isola...
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A number of uncouplers and energy transfer inhibitors suppress photosystem II cyclic photophosphorylation catalyzed by either a proton/electron or electron donor. Valinomycin and 2,4-dinitrophenol also inhibit photosystem II cyclic photophosphorylation, but these compounds appear to act as electron transport inhibitors rather than as uncouplers. Only when valinomycin, KCl, and 2,4-dinitrophenol...
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Isolated maize bundle sheath chloroplasts showed substantial rates of noncyclic photophosphorylation. A typical rate of phosphorylation coupled to whole-chain electron transport (methylviologen or ferricyanide as acceptor) was 60 mumol per hour per milligram chlorophyll) with a coupling efficiency (P/e(2)) of 0.6. Partial electron transport reactions driven by photosystem I or II supported phos...
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In spinach chloroplasts which have been shaken with n-heptane, photophosphorylation coupled to the reduction of nicotinamide adenine dinucleotide phosphate is inhibited about 95% while the photoreduction of NADP+ (and ferricyanide) is stimulated as much as 50%. Photophosphorylation catalyzed by phenazine methosulfate, however, is inhibited only 50%. These data are interpreted to indicate that t...
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The effects of disalicylidenepropanediamine (DSPD) and disulfo-disalicylidenepropanediamine (sulfo-DSPD) on the photosynthetic electron transport of isolated chloroplasts have been reexamined.Our data suggest that DSPD, but not sulfo-DSPD, is an effective inhibitor of electron transport between photosystem II and photosystem I before or at plastocyanin. Furthermore, both DSPD and sulfo-DSPD blo...
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ورودعنوان ژورنال:
- Biochimica et biophysica acta
دوره 333 2 شماره
صفحات -
تاریخ انتشار 1974