Bioluminescent nanosensors for protease detection based upon gold nanoparticle-luciferase conjugates.
نویسندگان
چکیده
This communication reports the use of click chemistry to site-specifically conjugate bioluminescent Renilla luciferase proteins to gold nanoparticles (Au NPs) for sensing protease activity. The bioluminescent emission from luciferase was efficiently quenched by Au NPs, but significantly recovered after the proteolytic cleavage.
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ورودعنوان ژورنال:
- Chemical communications
دوره 46 1 شماره
صفحات -
تاریخ انتشار 2010