Developing a microRNA body fluid identification test for use in forensic casework
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منابع مشابه
Applied Biosystems TaqMan MicroRNA Assays help researchers validate miRNA markers for forensic body fluid identification
Importance and challenges of identifying body fluids in forensic casework In forensic casework, correctly identifying body fluids such as blood, semen, or saliva is important for reconstructing a crime scene. Analysis of stains or biological fluids recovered during an investigation may provide potential sources of DNA that can be used to identify the donor of the fluid or stain [1]. However, bo...
متن کاملEvaluation of stem-loop reverse transcription and poly-A tail extension in microRNA analysis of body fluids.
MicroRNA has been demonstrated to be a viable tool for body fluid identification purposes in forensic casework. Stem-loop reverse transcription (slRT) is regularly used for cDNA synthesis from mature miRNA, along with poly-A tail extension. Both have been used in a forensic context, but no direct comparison has been carried out. It has also not been shown whether poly-A tail extension can be us...
متن کاملIdentification of forensically relevant body fluids using a panel of differentially expressed microRNAs.
The serology-based methods routinely used in forensic casework for the identification of biological fluids are costly in terms of time and sample and have varying degrees of sensitivity and specificity. Recently, the use of a molecular genetics-based approach using messenger RNA (mRNA) profiling has been proposed to supplant conventional methods for body fluid identification. However, the size ...
متن کاملBody fluid identification in forensics.
Determination of the type and origin of the body fluids found at a crime scene can give important insights into crime scene reconstruction by supporting a link between sample donors and actual criminal acts. For more than a century, numerous types of body fluid identification methods have been developed, such as chemical tests, immunological tests, protein catalytic activity tests, spectroscopi...
متن کاملConsidering the effect of stem-loop reverse transcription and real-time PCR analysis of blood and saliva specific microRNA markers upon mixed body fluid stains.
Forensic RNA analysis is gathering pace with reports of messenger RNA analysis being used in case work, and with microRNA being increasingly researched. Such techniques address a fundamental issue in body fluid identification, namely increased specificity over existing chemical tests, and the incorporation of additional body fluids such as vaginal material. The use of RNA analysis will be of pa...
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