Anti-Biofilm Performance of Three Natural Products against Initial Bacterial Attachment
نویسندگان
چکیده
Marine bacteria contribute significantly towards the fouling consortium, both directly (modern foul release coatings fail to prevent "slime" attachment) and indirectly (biofilms often excrete chemical cues that attract macrofouling settlement). This study assessed the natural product anti-biofilm performance of an extract of the seaweed, Chondrus crispus, and two isolated compounds from terrestrial sources, (+)-usnic acid and juglone, against two marine biofilm forming bacteria, Cobetia marina and Marinobacter hydrocarbonoclasticus. Bioassays were developed using quantitative imaging and fluorescent labelling to test the natural products over a range of concentrations against initial bacterial attachment. All natural products affected bacterial attachment; however, juglone demonstrated the best anti-biofilm performance against both bacterial species at a concentration range between 5-20 ppm. In addition, for the first time, a dose-dependent inhibition (hormetic) response was observed for natural products against marine biofilm forming bacteria.
منابع مشابه
Fluorescence-based quasicontinuous and in situ monitoring of biofilm formation dynamics in natural marine environments.
Analyzing the dynamics of biofilm formation helps to deepen our understanding of surface colonization in natural environments. While methods for screening biofilm formation in the laboratory are well established, studies in marine environments have so far been based upon destructive analysis of individual samples and provide only discontinuous snapshots of biofilm establishment. In order to exp...
متن کاملEvaluation of Anti biofilm and Antibiotic Potentiation Activities of Silver Nanoparticles Against some Nosocomial Pathogens
Nowadays silver nanoparticles (AgNPs) are used as antimicrobial due to its well known physical, chemical, and biological properties. A large collection of bacterial cells adhering to a surface is called bacterial biofilm. Exposure to silver nano particles (AgNPs) may prevent colonization of new bacteria onto the biofilm. In the present work, we have investigated whether the biofilm format...
متن کاملINHIBITORY EFFECT OF STEVIA AND ROSA EXTRACTS AGAINST BACTERIAL QUORUM SENSING
Background & Aims: Quorum Sensing is a mechanism by which orchestrate the expression of many genes in bacteria. Therefore, any interference with the system will inhibit bacterial infections. The principal purpose of the research was to evaluate the potential of anti-quorum sensing of Rosa damascena and Stevia rebaudiana against Staphylococcus aureus. Materials & Methods: Ten isolates of Staphy...
متن کاملAnti bacterial effects of nickel nano-particles on biofilm production amounts by B.capacia ATCC 25416
Background & Aims: B.cepacia is one of the causative agents of health care associated infections which have the ability of attachment to different surfaces and biofilm formation is one of the most important virulence factors in pathogenesis of this microorganism. Nanoparticles are key components which are considered for the designing of new antimicrobial agents, no studies have been done on the...
متن کاملIn vitro anti-biofilm activity of Quercus brantii subsp. persica on human pathogenic bacteria
Background and objectives: Quercus brantii subsp. persica is used in folk medicine to treat infections in Iran. There is not available report on the anti-biofilm activity of Quercus brantii subsp. persica. The aim of the present study was to investigate the effects of Quercus brantii subsp. persica against...
متن کامل