H-NS and genomic bridge building: lessons from the human pathogen Salmonella Typhi
نویسنده
چکیده
The H-NS protein has emerged as one of the leading causes of transcriptional repression in Gram-negative bacteria. In a paper published in this issue, De la Cruz and colleagues shed new light on the role of DNA curvature in the repressive mechanism, using a porin gene promoter from the human pathogen Salmonella Typhi as their experimental system (De la Cruz et al., 2009). Their data help to deepen our understanding of the importance of local DNA structure in facilitating the interaction of H-NS with its target sites in DNA and in establishing an effective nucleoprotein complex for the repression of transcription.
منابع مشابه
H-NS and genomic bridge building: lessons from the human pathogen Salmonella Typhi.
The H-NS protein has emerged as one of the leading causes of transcriptional repression in Gram-negative bacteria. In a paper published in this issue, De la Cruz and colleagues shed new light on the role of DNA curvature in the repressive mechanism, using a porin gene promoter from the human pathogen Salmonella Typhi as their experimental system (De la Cruz et al., 2009). Their data help to dee...
متن کاملAlterations in Gene Expression of Interferon and Tumor Necrosis Factor‐α in Human Blood Macrophage-Like Monocytes Induced by Clinical and Standard Salmonella typhi Strains in vitro
Background and Objective: Salmonella typhi as a human pathogen stimulates the human immune system and triggers gene expression changing its pathogenesis. Therefore, we aimed to investigate the expression levels of ifn-γ and tnf-α cytokines in human blood macrophage-like monocytes in dealing with clinical and standard samples of Salmonella typhi in vivo. Materials and Methods: In this cross-sec...
متن کاملGenomic subtractive hybridization and selective capture of transcribed sequences identify a novel Salmonella typhimurium fimbrial operon and putative transcriptional regulator that are absent from the Salmonella typhi genome.
Salmonella typhi, the etiologic agent of typhoid fever, is adapted to the human host and unable to infect nonprimate species. The genetic basis for host specificity in S. typhi is unknown. The avirulence of S. typhi in animal hosts may result from a lack of genes present in the broad-host-range pathogen Salmonella typhimurium. Genomic subtractive hybridization was successfully employed to isola...
متن کاملGenome subtraction for novel target definition in Salmonella typhi
Large genomic sequencing projects of pathogens as well as human genome leads to immense genomic and proteomic data which would be very beneficial for the novel target identification in pathogens. Subtractive genomic approach is one of the most useful strategies helpful in identification of potential targets. The approach works by subtracting the genes or proteins homologous to both host and the...
متن کاملPrediction of Toxin-Antitoxin system (TA system) as a Novel Potent Target in Salmonella typhi Using Bioinformatics Analysis
Background and Objective: Salmonella typhi is one of the major challenges for the human and animal health. Salmonella with high pathogenicity can be harmful factor for human health. The control of this pathogen is a big challenge as it can cause serious infectious diseases such as gastroenteritis, septicemia and typhoid fever. On the other side, there are many factors such as toxin-antitoxin (T...
متن کامل