Quantitative analysis of phospholipids by thin-layer chromatography.

A method for microdetermination of major neutral lipids and an application of the procedure to tissue lipids.

Cholesterol, cholesteryl esters, triglycerides and fatty acids as major neutral lipids and phospholipids were examined in quantitative analysis. The method consisted of three steps: (1) separation of lipids by one-dimensional thin-layer chromatography on silica gel plates; (2) elution of neutral lipids from scraped silica gel with chloroform-methanol (4:1); and (3) colorimetric determination of...

Quantitative Two-dimensional Thin-layer Chromatography of Naturally Occurring Phospholipids.

Separation of phosphatidylinositols and other phospholipids by two-step one-dimensional thin-layer chromatography.

A quick and efficient thin-layer chromatographic procedure is described for the separation of phosphatidylinositol-4,5-bisphosphate, phosphatidylinositol-4-monophosphate, phosphatidylinositol, phosphatidylcholine, phosphatidic acid, and phosphatidylethanolamine. The method involves development of the phospholipids successively in two different solvent systems but in the same direction. The meth...

Rapid quantitative measurement of lung tissue phospholipids.

A rapid procedure for the separation of phospholipids of lung tissue into acidic and nonacidic fractions by means of diethylaminoethyl cellulose acetate microcolumns is described. The fractions are then resolved into individual phospholipids by thin-layer chromatography and quantified by transmission densitometry.

Isolation and Assay of Surface-active Phospholipid Components from Lung Extracts by Thin-layer Chromatography.

The isolation and quantitation of the surface-active phospholipids of the lipids extracted from mammalian saline lung extracts were studied by the direct application of thin-layer chromatography. The significant recovery of 85% of the phospholipids suggests that the method described can be used for the quantitative analysis of the surface-active material of lung extract and for further compleme...

New colorimetric method for the quantitative estimation of phospholipids without acid digestion.

A unique colorimetric method for the quantitative determination of phospholipids that does not involve the acid digestion of the lipid is described. The phospholipids, after separation by thin-layer chromatography and elution from the silica gel, are heated with a chromogenic solution that is a modification of a spray reagent formulated by Vaskovsky and Kostetsky (1968, J. Lipid Res., 9: 396). ...