The effect of natural adjuvants (G2, G2F) on lung inflammation of sensitized guinea pigs

OBJECTIVE The effects of natural adjuvants were examined on total and differential WBC counts in lung lavage of sensitized guinea pigs. MATERIALS AND METHODS In three sensitized groups of guinea pigs including: untreated sensitized animals (S), sensitized animals treated with adjuvant G2 (S+G2) and G2F (S+G2F) as well as non-sensitized group (C) (n=6 for each group), total and differential WBC counts of lung lavage were examined. Sensitization of animals was achieved by injection and inhalation of ovalbumin (OA). RESULTS The results showed increased total WBC, eosinophil, neutrophil, and basophil counts, and decreased lymphocytes in lung lavage of sensitized animals compared with the control group (p<0.01 for all cases). However, neutrophil, lymphocyte, eosinophil, and basophil counts in lung lavage were decreased in treated groups with either G2 or G2F but total WBC was decreased in lung lavage of treated group with only G2. CONCLUSION These results indicate important preventive effects of two natural adjuvants, especially G2, on lung inflammation of sensitized guinea pigs.