High performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS-MS) for the quantification of L-kynurenine and indole-3-acetic acid in grape must by isotope dilution assay
نویسندگان
چکیده
S u m m a r y : Isotope dilution assay was used for the qualitative and quantitative analysis of L-kynurenine and indole-3-acetic acid in grape must . After solid phase extraction, highest selectivity and sensitivity was achieved by high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS-MS) using selected reaction monitoring (SRM) . In the 24 samples under study, the amounts of L-kynurenine and indole-3-acetic acid ranged from 0 to 94 ~tg~lI and from 20 to 380 Ilg~l-1 , respectively . These compounds are considered as potential precursors of 2-aminoacetophenone ; causing the "untypical aging offflavour" in Vitis vinifera white wines .
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