Study the synergistic effect of collagen type I, hyaluronic acid and chitosan on adhesion, proliferation and osteoblast differentiation of bone marrow derived human mesenchymal stem cells
ثبت نشده
چکیده
The Extra cellular matrix (ECM) components are considered as good candidates for fabricating scaffolds with superior bioactivity or functionalizing biomaterials to improve their performance (Green, 2008). A biomimetic surface coating to improve or enhance the osteoconductivity of biomaterials and tissue engineering scaffolds holds great promise in orthopaedic and bone tissue engineering applications.
منابع مشابه
A novel tripolymer coating demonstrating the synergistic effect of chitosan, collagen type 1 and hyaluronic acid on osteogenic differentiation of human bone marrow derived mesenchymal stem cells.
The biomimetic approach mimicking in vivo micro environment is the key for developing functional tissue engineered constructs. In this study, we used a tripolymer combination consisting of a natural polymer, chitosan and two extracellular matrix components; collagen type 1 and hyaluronic acid to coat tissue culture plate to evaluate their effect on osteogenic differentiation of human bone marro...
متن کاملComparison of Proliferation and Osteoblast Differentiation of Marrow-Derived Mesenchymal Stem Cells on Nano- and Micro-Hydroxyapatite Contained Composite Scaffolds
Bones constructed by tissue engineering are being considered as valuable materials to be used for regeneration of large defects in natural bone. In an attempt to prepare a new bone construct, in this study, proliferation and bone differentiation of marrow-derived mesenchymal stem cells (MSCs) on our recently developed composite scaffolds of nano-, micro-hydroxyapatite/ poly(l-lactic acid) were ...
متن کاملMatrigel Enhances in vitro Bone Differentiation of Human Marrow-derived Mesenchymal Stem Cells
Objective(s) The use of co-culture cells as well as extra cellular matrix are among those strategies that have been employed to direct mesenchymal stem cell (MSC) bone differentiation in culture. In this regard, there is no study considering the effects of Matrigel on mesenchymal stem cell (MSC) in vitro bone differentiation. This was the subject of the present study. Materials and Methods ...
متن کاملEffect of Lithium Chloride on Proliferation and Bone Differentiation of Rat Marrow-Derived Mesenchymal Stem Cells in Culture
Objective(s) It is believed that the mesenchymal stem cell (MSC) differentiation and proliferation are the results of activation of wnt signaling pathway. On the other hand, lithium chloride is reported to be able to activate this pathway. The objective of this study was to investigate the effect of lithium on in vitro proliferation and bone differentiation of marrow-derived MSC. Materials and ...
متن کاملEffects of BIO on proliferation and chondrogenic differentiation of mouse marrow-derived mesenchymal stem cells
In vitro expansion of mesenchymal stem cell (MSCs) into large number is necessary for their application in cell-based treatment of articular cartilage defects. On the other hand, some studies have indicated that BIO (6-Bromoindirubin-3-Oxime) possesses mitogenic effects on cell culture. The objective of the present study was to examine the effect of BIO on in vitro expansion and chondrogenic di...
متن کامل