13C MRS Studies of the Control of Hepatic Glycogen Metabolism at High Magnetic Fields
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Technical and experimental features of Magnetic Resonance Spectroscopy of brain glycogen metabolism.
In the brain, glycogen is a source of glucose not only in emergency situations but also during normal brain activity. Altered brain glycogen metabolism is associated with energetic dysregulation in pathological conditions, such as diabetes or epilepsy. Both in humans and animals, brain glycogen levels have been assessed non-invasively by Carbon-13 Magnetic Resonance Spectroscopy (13C-MRS) in vi...
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Carbon-13 magnetic resonance spectroscopy (13C MRS) offers a noninvasive method to assess glycogen levels in skeletal muscle and to identify excess glycogen accumulation in patients with glycogen storage disease (GSD). Despite the clinical potential of the method, it is currently not widely used for diagnosis or for follow-up of treatment. While it is possible to perform acceptable 13C MRS at l...
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To determine the effect of insulin-dependent diabetes mellitus (IDDM) on rates and pathways of hepatic glycogen synthesis, as well as flux through hepatic pyruvate dehydrogenase, we used 13C-nuclear magnetic resonance spectroscopy to monitor the peak intensity of the C1 resonance of the glucosyl units of hepatic glycogen, in combination with acetaminophen to sample the hepatic UDP-glucose pool ...
متن کاملDirect validation of in vivo localized 13C MRS measurements of brain glycogen.
With the use of localized 13C MRS in conjunction with [1-(13)C]-D-glucose infusion, it is possible to study brain glycogen metabolism in vivo. The purpose of this study was to validate in vivo 13C MRS measurements by comparing them with results from a standard biochemical assay. To increase the [1-(13)C] glycogen concentration, 11 rats were subjected to an episode of acute hypoglycemia followed...
متن کاملStimulatory effect of glucokinase activation on hepatic glycogen turnover as measured by 13C-MRS
Introduction Glucokinase (GK), a rate controlling enzyme present in both the liver and the pancreas, acts as a glucose sensor by facilitating the phosphorylation of glucose to glucose-6-phosphate (G6P). In response to rising levels of plasma glucose, GK activation triggers insulin secretion by the pancreatic β-cells and causes a shift in glucose metabolism towards storage and utilization in the...
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