Structural biology of starch-degrading enzymes and their regulation.
نویسندگان
چکیده
Starch is a major energy source for all domains of life. Recent advances in structures of starch-degrading enzymes encompass the substrate complex of starch debranching enzyme, the function of surface binding sites in plant isoamylase, details on individual steps in the mechanism of plant disproportionating enzyme and a self-stabilised conformation of amylose accommodated in the active site of plant α-glucosidase. Important inhibitor complexes include a flavonol glycoside, montbretin A, binding at the active site of human pancreatic α-amylase and barley limit dextrinase inhibitor binding to the debranching enzyme, limit dextrinase using a new binding mode for cereal protein inhibitors.
منابع مشابه
SCREENING FOR STARCH-HYDROLYSING BACTERIA
Screening of3000 soil samples collected from cities of four different provinces of Iran for starch-hydrolysing bacteria revealed that the nature is enriched with Streptomyces species capable of producing amylolytic enzymes. Among the bacterial isolates, one of the high starch-degrading strains was selected for further microbiological identification and also amylolytic enzyme(s) characteriza...
متن کاملThe extracellular enzymes of Coniophora cerebulla.
1. The extracellular enzymes present in the culture filtrates of Coniophora cerebella grown on various carbohydrate carbon sources were investigated. Enzymes that degraded cellulose derivatives, hemicellulose fractions, starch, laminarin, pectin and several oligosaccharides were detected. 2. All the polysaccharide-degrading activities were adaptive except for that acting on laminarin. 3. The cu...
متن کاملIn vitro utilization of amylopectin and high-amylose maize (Amylomaize) starch granules by human colonic bacteria.
It has been well established that a certain amount of ingested starch can escape digestion in the human small intestine and consequently enters the large intestine, where it may serve as a carbon source for bacterial fermentation. Thirty-eight types of human colonic bacteria were screened for their capacity to utilize soluble starch, gelatinized amylopectin maize starch, and high-amylose maize ...
متن کاملRegulation of starch accumulation by granule-associated plant 14-3-3 proteins.
In higher plants the production of starch is orchestrated by chloroplast-localized biosynthetic enzymes, namely starch synthases, ADP-glucose pyrophosphorylase, and starch branching and debranching enzymes. Diurnal regulation of these enzymes, as well as starch-degrading enzymes, influences both the levels and composition of starch, and is dependent in some instances upon phosphorylation-linked...
متن کاملReview of Literature
Amylases are starch degrading enzymes. They are widely distributed in microbial, plant and animal kingdoms (Banks et al, 1975). They degrade starch and related polymers to yield products characteristic of individual amylolytic enzymes. Initially the term amylase was used originally to designate enzymes capable of hydrolysing α-1, 4glycosidic bonds of amylose, amylopectin, glycogen and their deg...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Current opinion in structural biology
دوره 40 شماره
صفحات -
تاریخ انتشار 2016