BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Pages 499-505 ISOLATION AND CHARACTERIZATION OF A NOVI~ CYI'OCItROME P-450-LIKE PSEUDOGENE

A r a b b i t l i v e r P 4 5 0 1 i k e p s e u d o g e n e h a s b e e n i s o l a t e d f rom a Z phage genomic l i b r a r y . Sequence a n a l y s i s r e v e a l e d s t r u c t u r a l homology w i t h r e s p e c t t o t h e r a t IX-450b and P--450e g e n e s as w e l l as a s i m i l a r i n t r o n e x o n o r g a n i z a t i o n . A 5 P p r o x i m a l TATA b o x l i k e s e q u e n c e and two 3 * d i s t a l p u t a t i v e p o l y a d e n y l a t i o n s i g n a l s were i d e n t i f i e d , and a l l p u t a t i v e i n t r o n e x o n b o u n d a r i e s e x c e p t a t t h e 3 W s p l i c e s i t e o f i n t r o n 2 w e r e f o u n d t o f o l l o w t h e GT/AG r u l e . Wi th a l l o w a n c e f o r a p p a r e n t d e l e t i o n s and i n s e r t i o n s , t h e s t r u c t u r a l h o m o l o g y o f t h e a m i n o a c i d s e q u e n c e d e d u c e d f r o m t h e p s e u d o g e n e w i t h r e s p e c t t o r a b b i t P 4 5 0 i s o z y m e 2 i s l o w e r f o r e x o n s 1 t h r o u g h 4 ( 1 8 2 8 ~ ) t h a n f o r exons 5 t h r o u g h 9 ( 4 2 6 5 ~ ) . S I n u c l e a s e mapp ing showed t h a t mRNAs c o m p l e m e n t a r y t o t h e I)NA s e q u e n c e of exon 9 a r e e x p r e s s e d . H o w e v e r , due t o t h e a l t e r a t i o n s i n t h e p s e u d o g e n e , i t a p p e a r s t h a t f u n c t i o n a l P-450 would n o t be p r o d u c e d f rom such mRNAs. © 1986 Academic Press, I~c