Automated and semi-automated screening, LCMSMS confirmation and human hepatocyte high-resolution metabolism studies of synthetic cannabinoids
نویسندگان
چکیده
Methods Homogeneous (Immunalysis), and heterogeneous (Randox; National Medical Services (NMS)) screening assays were evaluated with authentic urine specimens. An LCMSMS qualitative confirmation method for synthetic cannabinoids (JWH-018, JWH-073, JWH-081, JWH-122, JWH-200, JWH-210, JWH-250, AM 2201 and RCS-4) and 20 metabolites in human urine was developed. Specimens were hydrolyzed and protein precipitated, followed by single MRM transition monitoring or survey scan that triggered an enhanced product ion scan at 3 different collision energies on the ABSciex 5500 Qtrap.
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