VEGF and ATP act by different mechanisms to increase microvascular permeability and endothelial [Ca]i
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چکیده
Pocock, T. M., B. Williams, F. E. Curry, and D. O. Bates. VEGF and ATP act by different mechanisms to increase microvascular permeability and endothelial [Ca]i. Am J Physiol Heart Circ Physiol 279: H1625–H1634, 2000.—Vascular endothelial growth factor (VEGF) increases hydraulic conductivity (Lp) by stimulating Ca 21 influx into endothelial cells. To determine whether VEGF-mediated Ca influx is stimulated by release of Ca from intracellular stores, we measured the effect of Ca store depletion on VEGF-mediated increased Lp and endothelial intracellular Ca concentration ([Ca]i) of frog mesenteric microvessels. Inhibition of Ca influx by perfusion with NiCl2 significantly attenuated VEGF-mediated increased [Ca]i. Depletion of Ca stores by perfusion of vessels with thapsigargin did not affect the VEGF-mediated increased [Ca]i or the increase in Lp. In contrast, ATP-mediated increases in both [Ca]i and Lp were inhibited by thapsigargin perfusion, demonstrating that ATP stimulated store-mediated Ca influx. VEGF also increased Mn influx after perfusion with thapsigargin, whereas ATP did not. These data showed that VEGF increased [Ca]i and Lp even when Ca 21 stores were depleted and under conditions that prevented ATP-mediated increases in [Ca]i and Lp. This suggests that VEGF acts through a Ca store-independent mechanism, whereas ATP acts through Ca store-mediated Ca influx.
منابع مشابه
VEGF and ATP act by different mechanisms to increase microvascular permeability and endothelial [Ca(2+)](i).
Vascular endothelial growth factor (VEGF) increases hydraulic conductivity (L(p)) by stimulating Ca(2+) influx into endothelial cells. To determine whether VEGF-mediated Ca(2+) influx is stimulated by release of Ca(2+) from intracellular stores, we measured the effect of Ca(2+) store depletion on VEGF-mediated increased L(p) and endothelial intracellular Ca(2+) concentration ([Ca(2+)](i)) of fr...
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