Prevalence of ESBL and MBL in Acinetobacter Species Isolated from Clinical Samples in Tertiary Care Hospital

Objective: The emergence of infections by Acinetobacter spp causes serious threats and further limits the treatment options. The present study aims to determine prevalence of ESBL and MBL among Acinetobacter species. Materials and methods: Various clinical specimens and isolates were identified using a standard bacteriological protocol. Antimicrobial sensitivity patterns were determined using Kirby Bauer disc diffusion method. ESBL was detected by phenotypic confirmatory disc diffusion test (PCDDT) using ceftazidime alone and ceftazidime in combination with clavulanic acid. MBL detection was done by Imipenem EDTA combined disc diffusion test. Result and Conclusion: The incidence of Acinetobacter was more in male( 64.17% ) than female (35.82). Among the males 16-40 years age group was more prevalent (43.28%). Out of the total 67 Acinetobacter spp maximum number of isolates were sensitive to imipenem ( 90%), followed by meropenem(87.57%) and amikacin (80.21%). Out of total 67 isolates 34(50.70%) were ESBL producer and 16 (23.88%) were MBL producer. Early detection of beta lactamase producing Acinetobacter strains would be important for better patient outcome including reduction of mortality rates and spread of multidrug resistant strains.