FOUR DIFFERENTIALLY EXPRESSED cDNAs CONTAINING THE REBERS- RIDDIFORD CONSENSUS SEQUENCE IN CALLINECTES SAPIDUS
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چکیده
.................................................................................................................................. iv ACKNOWLEDGMENTS ...............................................................................................................v LIST OF FIGURES ....................................................................................................................... vi LIST OF TABLES........................................................................................................................ vi
منابع مشابه
Differential expression of eight transcripts and their roles in the cuticle of the blue crab, Callinectes sapidus.
Eight cuticle protein transcripts from Callinectes sapidus were sequenced and their expression determined across the molt cycle in both calcifying and arthrodial cuticle hypodermis using quantitative PCR, Northern blots, and in situ hybridization. Four transcripts, designated CsAMP, are found only in non-calcifying arthrodial membrane hypodermis. They all code for a Rebers-Riddiford-1 motif, kn...
متن کاملIdentifying exoskeleton proteins in the blue crab from an expressed sequence tag (EST) library.
A blue crab (Callinectes sapidus) expressed sequence tag project was designed for multiple purposes including discovery of genes for cuticular (exoskeletal) proteins, some of which may regulate mineralization. One of the expression libraries sequenced was from the hypodermis (the epithelium depositing the cuticle). RNAs used for cDNA synthesis were pooled from arthrodial and mid-dorsal hypoderm...
متن کاملIdentification and developmental expression of mRNAs encoding crustacean cardioactive peptide (CCAP) in decapod crustaceans.
Full-length cDNAs encoding crustacean cardioactive peptide (CCAP) were isolated from several decapod (brachyuran and astacuran) crustaceans: the blue crab Callinectes sapidus, green shore crab Carcinus maenas, European lobster Homarus gamarus and calico crayfish Orconectes immunis. The cDNAs encode open reading frames of 143 (brachyurans) and 139-140 (astacurans) amino acids. Apart from the pre...
متن کاملCloning of aquaporin-1 of the blue crab, Callinectes sapidus: its expression during the larval development in hyposalinity
BACKGROUND Ontogenetic variation in salinity adaptation has been noted for the blue crab, Callinectes sapidus, which uses the export strategy for larval development: females migrate from the estuaries to the coast to spawn, larvae develop in the ocean, and postlarvae (megalopae) colonize estuarine areas. We hypothesized that C. sapidus larvae may be stenohaline and have limited osmoregulatory c...
متن کاملStructural determination and functional analysis of a soluble matrix protein associated with calcification of the exoskeleton of the crayfish, Procambarus clarkii.
A novel matrix protein named calcification-associated soluble protein-2 (Casp-2) was isolated from the acetic acid-soluble fraction of the exoskeleton of the crayfish Procambarus clarkii, and its primary structure was determined by a combination of peptide sequencing, mass spectral analysis, and cDNA cloning. Casp-2 consists of 117 amino acid residues and has a chitin-binding consensus sequence...
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