A Study on the Use of Α-amylase Inhibitor from Vigna Unguiculata Seeds as an Organic Nitrogen Source in Plant Tissue Culture
نویسندگان
چکیده
α-Amylase inhibitor from Vigna unguiculata seeds (VUAI) was purified using ionexchange chromatography on Poros HS-50 column. Plant cell culture was used as it provides a controlled environmental and nutritional condition. VUAI used in different concentrations as 10, 100 and 200 ppm was found to increase the biomass of both grape and peanut callus cells in liquid media. Increased anthocyanin content in grape callus and total phenolics content in peanut callus was observed in VUAI treated groups. The VUAI treated groups showed better antioxidant activities than the control group. The SDS-PAGE profile showed a marked increase in the number of secreted proteins in the VUAI treated groups.
منابع مشابه
Characterization of the Α-amylase Inhibitor from the Seeds of Macrotyloma Uniflorum and Vigna Unguiculata
α-amylase inhibitors were purified and characterized from the seeds of Macrotyloma uniflorum (MUAI) and Vigna unguiculata (VUAI). MUAI was purified using CM-cellulose column while VUAI was purified using Poros HS-50 column followed by gel filtration on Sephadex G-75 column. The molecular weights of MUAI and VUAI as determined by gel filtration were 26.91 kD and 21.89 kD respectively. Both MUAI ...
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