Genetic Polymorphic Study of Αs2-casein Gene in Different Breeds of Buffaloes
نویسندگان
چکیده
The present investigation was aimed at studying 1.26 kb αS2-casein promoter gene in αS2casein gene locus between the three buffalo breeds. αS2 -casein promoter gene was amplifi ed by PCR using oligonucleotide primers standardized for Bos taurus species. The sizes of the amplifi cation products were similar. PCR amplicons are subjected to restriction digestion by the enzyme Eco RV, only an intact PCR fragments were observed in all the breeds studied. No variation was found when the Same PCR amplicons of αS2-casein promoter gene subjected to nucleotide sequence analysis. The BLAST alignment of 1262 bp nucleotides of buffalo αS2-casein gene showed homogeneity with the Bos taurus and Bos grunniens αS2-casein gene. The milk components and milk yield parameters could not be associated with buffalo αS2-casein genotypes due to their monomorphic haplotype.
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تاریخ انتشار 2013