Introduction P Application Note
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چکیده
Introduction Measurement of volumetric properties of biopolymers in dilute solution has always been a difficult procedure, involving tedious experiments using densitometric or dilatometric methods to determine partial molar volumes. Measuring volume changes (∆Vunf) for biopolymer unfolding is also problematic and most often carried out using large, reinforced optical cells up to pressures of 10,000 atm. These limitations have led to a dearth of volumetric data in the literature, and a recognition that a meaningful understanding of the relationship between structure and volume is lacking. MicroCal has recently developed (patent pending) a calorimetric technique, Pressure Perturbation Calorimetry (PPC), which not only eases the experimental burden but also leads to considerably greater accuracy for measuring critical volumetric parameters of biopolymers in solution. Equally significant, it provides information on biopolymer solvation currently unavailable from any other method.
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