Identification and molecular characterization of Van A-type vancomycin-resistant Enterococcus faecalis in Northeast of Brazil.

نویسندگان

  • Marinalda Anselmo Vilela
  • Sandra Lavareda de Souza
  • Izabel Cristina Vanzato Palazzo
  • Joseane Cristina Ferreira
  • Marcos Antonio de Morais
  • Ana Lúcia da Costa Darini
  • Márcia Maria Camargo de Morais
چکیده

The isolation of vancomycin resistant enterococci (VRE) in Brazil has rapidly increased, following the world wide tendency. We report in the present study the first isolation of vancomycin resistant Enterococcus faecalis (VRE) in the Northeast of Brazil. The four VRE isolates were characterized for antimicrobial susceptibility, genotypic typing by macro restriction of chromosomal DNA followed by pulsed-field gel electrophoresis and for characterization of the Tn1546-like element and plasmid contents. The isolates showed resistance to multiple antibiotics and a single genotype profile, suggesting the dissemination of a single clone among the patients. Tn1546 associated to genetic elements as plasmids shows the importance of infection control measures to avoid the spreading of glycopeptide resistance by conjugative transfer of VanA elements.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Evaluation of a PCR Assay to Detect Enterococcus faecalis in Blood and Determine Glycopeptides Resistance Genes: Van A and Van B

Background: Bacteremia due to Enterococcus faecalis is usually caused by strains resistant to most antibiotics. Effective management of the disease is dependent on rapid detection and characterization of the bacteria, and determination its sensitivity pattern to antimicrobial drugs. The aim of this study was to investigate a more rapid and reliable assay for simultaneous diagnosis of enterococc...

متن کامل

Evaluation of drug sensitivity of Entrococcus faecium and Entrococcus faecalis Strains and Detection of VanA/B Genes in Vancomycin Resistance Isolated by PCR Method

Enterococci, as the major cause of nosocomial infections, have been shown to have an increase in drug resistance during the past two decades. TheEntrococcalemergence of Vancomycin-resistant is very important nowadays. The purpose of this study was to determine the drug sensitivity of Enterococcus faecalis and Enterococcus faecium species against Vancomycin, Tetracycline, Gentamicin, Erythromyci...

متن کامل

Molecular Identification of Pathogenic Enterococci and Evaluation of Multi-drug Resistance in Enterococcus Species Isolated From Clinical samples of Some Hospitals in Tehran, Iran

Background: Multidrug-resistant (MDR) enterococci cause many problems for physicians and infection control specialists in the recent years. Hence, by the identification of antibiotic resistance patterns of enterococci in different geographical regions an appropriate strategy can be developed to prevent bacterial antibiotic resistance and provide effective treatment. The current study aimed at i...

متن کامل

Epidemiologic evaluation of Vancomycin Resistant genes in Enterococcus spp. isolated from clinical samples

Background & Objectives: Isolation of vancomycin resistant Enterococcus from clinical samples is very important. The aim of this study was evaluation of phenotype and genotype of van genes in vancomycine resistant Enterococcus. Materials and Methods: 411 Enterococcus isolates were collected from selected Tehran’s hospitals between March 2004 and December 2007. The enterococcal isolates were ide...

متن کامل

First report of vancomycin-resistant enterococcus faecalis in Uberaba, Minas gerais state

In this study we report the first isolation of VanA-type vancomycin-resistant Enterococcus faecalis strains from two different patients hospitalized in the same intensive care unit at the hospital of Universidade Federal do Triângulo Mineiro (UFTM), Uberaba, Minas Gerais, Brazil.

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Memorias do Instituto Oswaldo Cruz

دوره 101 7  شماره 

صفحات  -

تاریخ انتشار 2006