Circulating Immunoglobulin - Secreting Cells in Patients With Plasma Cell
نویسندگان
چکیده
Immunoglobulin-secreting cells (ISC) in peripheral blood mononuclear cells (PBM) isolated from patients with plasma cell dyscrasia of various stages were studied using reverse hemolytic plaque assay. Normal healthy individuals contained 35 ± 16 ISC/105 PBM. Aleukemic or subleukemic patients with overt myeloma contained 825 ± 713 ISC. whereas leukemic patients contained 1 2,675 ± 2520 ISC. Patients of premyelomatous stage. however. contained ISC I 1 0 PBM of normal range. The relationship between ISC and clinical stage or laboratory data is discussed.
منابع مشابه
A plaque assay to enumerate circulating Ig-secreting cells of each type of the different Ig classes.
A new method to quantitate circulating cells secreting immunoglobulin (Ig) of each type of the different classes by hemolytic plaque technique is reported. The ratio of kappa (k)-secreting cells and lambda(lambda)-secreting cells of the different lg classes in normal individuals is almost equal to that of kappa:lambda light chains in corresponding serum lg class, whereas the ratio in plasma cel...
متن کاملCirculating immunoglobulin-secreting cells in patients with plasma cell dyscrasia.
Immunoglobulin-secreting cells (ISC) in peripheral blood mononuclear cells (PBM) isolated from patients with plasma cell dyscrasia of various stages were studied using reverse hemolytic plaque assay. Normal healthy individuals contained 35 +/- 16 ISC/10(5) PBM. Aleukemic or subleukemic patients with overt myeloma contained 825 +/- 713 ISC, whereas leukemic patients contained 12,675 +/- 2520 ISC...
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