Electrophysiological Recordings Using Spatially Arranged Microelectrode Probes Embedded into 3-d Neuronal Cultures
نویسندگان
چکیده
This paper presents spatially arranged microelectrode probes to allow electrophysiological recordings inside 3–D neuronal cultures. Out–of–plane microelectrode probes standing on a substrate have gradation in height. Wire– bonding–based probe technology [1] makes it possible to provide the flexible probes. We would understand the dependent alteration of cellular activities caused by the construction process of 3–D artificial neuronal networks, because the developed probes can directly and spatially record cellular activities inside 3–D cell cultures. This paper demonstrates electrophysiological activities inside 3–D neuronal cultures could be successfully recorded using the embedded probes.
منابع مشابه
Implantable microelectrode arrays for simultaneous electrophysiological and neurochemical recordings.
Implantable microfabricated microelectrode arrays represent a versatile and powerful tool to record electrophysiological activity across multiple spatial locations in the brain. Spikes and field potentials, however, correspond to only a fraction of the physiological information available at the neural interface. In urethane-anesthetized rats, microfabricated microelectrode arrays were implanted...
متن کاملMulti-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures
The cortex is spontaneously active, even in the absence of any particular input or motor output. During development, this activity is important for the migration and differentiation of cortex cell types and the formation of neuronal connections. In the mature animal, ongoing activity reflects the past and the present state of an animal into which sensory stimuli are seamlessly integrated to com...
متن کاملChallenges and opportunities for large-scale electrophysiology with Neuropixels probes
Electrophysiological methods are the gold standard in neuroscience because they reveal the activity of individual neurons at high temporal resolution and in arbitrary brain locations. Microelectrode arrays based on complementary metal-oxide semiconductor (CMOS) technology, such as Neuropixels probes, look set to transform these methods. Neuropixels probes provide ∼1000 recording sites on an ext...
متن کاملRecording Large-scale Neuronal Ensembles with Silicon Probes in the Anesthetized Rat
Large scale electrophysiological recordings from neuronal ensembles offer the opportunity to investigate how the brain orchestrates the wide variety of behaviors from the spiking activity of its neurons. One of the most effective methods to monitor spiking activity from a large number of neurons in multiple local neuronal circuits simultaneously is by using silicon electrode arrays. Action pote...
متن کاملAntibiotic Supplements Affect Electrophysiological Properties and Excitability of Rat Hippocampal Pyramidal Neurons in Primary Culture
Introduction: Antibiotic supplements are regularly used in neuronal culture media to control contamination however, they can interfere with the neuronal excitability and affect electrophysiological properties. Therefore, in this study, the effect of penicillin/streptomycin supplements on the spontaneous electrophysiological activity of hippocampal pyramidal neurons was examined. Methods: Electr...
متن کامل